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Abstract：
Despite the advent of molecular assessment, banding cytogenetics and fluorescence in situ hybridization (FISH) still have a significant role in diagnostic and prognostic approaches to chronic myeloid leukaemia (CML). Area covered: At diagnosis and during treatment with tyrosine kinase inhibitors (TKIs), cytogenetics is used to detect the Philadelphia chromosome, with its typical translocation t(9;22)(q34;q11.2), and any additional or other chromosomal aberrations (ACAs and OCAs) that may arise in 5-10% of cases, the latter associated to transformation of the disease in blast phases. In this review, the potential role of banding cytogenetics and FISH is discussed through a review of published papers on the prognostic impact of these tools in CML treatment and monitoring. Expert commentary: Cytogenetic techniques, including banding cytogenetics and FISH, continue to maintain a crucial role in CML monitoring. At diagnosis and after 3 months of therapy, banding cytogenetics will continue to be an essential test to perform, but it will become redundant after the achievement of a major molecular response (MMR) assessed with molecular techniques. FISH analysis maintains its usefulness in monitoring the response to TKIs only in special situations.
摘要：
尽管分子评估的出现，条带化细胞遗传学和荧光原位杂交(FISH)在慢性髓性白血病(CML)的诊断和预后方法中仍有重要作用.涵盖的领域：在诊断和酪氨酸激酶抑制剂（TKIs）治疗期间，细胞遗传学用于检测费城染色体，其典型易位t(9；22)(q34；q11.2)，以及在5-10%的病例中可能出现的任何额外或其他染色体畸变（ACA和OCA），后者与爆炸阶段的疾病转化有关。在这次审查中,我们通过对这些工具在CML治疗和监测中的预后影响的已发表论文进行综述,讨论了细胞遗传学和FISH条带的潜在作用.专家评论：细胞遗传学技术，包括条带细胞遗传学和FISH，继续在CML监测中发挥关键作用。在诊断时和治疗3个月后，条带细胞遗传学将继续是一项必不可少的测试，但它将成为多余后，主要分子反应（MMR）的分子技术评估的成就。FISH分析仅在特殊情况下在监测对TKI的响应方面保持其有用性。
