1H, 13C and 15N NMR chemical shift assignments of A. thaliana RCD1 RST.

1H,拟南芥 RCD1RST 的 13C 和 15NNMR 化学位移分配。

• 影响指数: 0.731
• 发表时间: Oct 2017
• 来源期刊: Biomol NMR Assign
• DOI: 10.1007/s12104-017-9749-4
• PMID: 28593560
• 文章类型: Journal Article
• 作者列表: Tossavainen H,Hellman M,Vainonen JP,Kangasjärvi J,Permi P

关键词: NMR assignments; RCD1; RST

Mesh: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular; Nuclear Proteins / chemistry; Protein Domains

Abstract

The A. thaliana RCD1 (radical-induced cell death1) protein is a cellular signaling hub protein which interacts with numerous plant transcription factors from different families. It consists of three conserved domains and intervening unstructured regions, the C-terminal RST domain being responsible for the interactions with the transcription factors. It has been shown that many partner proteins interact with RCD1 RST via their intrinsically disordered regions, and that the domain is able to house partners with divergent folds. We aim to structurally characterize the RCD1 RST domain and its complexes [complex with DREB2A]. Here we report the 1H, 15N and 13C chemical shift assignments of the backbone and sidechain atoms for RCD1 (468-589) containing the RST (510-567) domain.

摘要

拟南芥RCD1（自由基诱导的细胞死亡1）蛋白是一种细胞信号传导中心蛋白，与来自不同家族的许多植物转录因子相互作用。它由三个保守域和中间的非结构化区域组成，C端RST结构域负责与转录因子的相互作用。已经表明，许多伴侣蛋白通过其固有的无序区域与RCD1RST相互作用，并且该领域能够容纳具有不同折叠的合作伙伴。我们旨在在结构上表征RCD1RST结构域及其复合物[与DREB2A的复合物]。在这里我们报告1H，含有RST(510-567)结构域的RCD1(468-589)的骨架和侧链原子的15N和13C化学位移分配。