Abstract：
Selenium is an essential trace and there is a high selenium concentration in the thyroid gland. Selenium deficiency may impair the thyroid function. The aim of this study was to investigate the association between three selenoprotein genes polymorphisms and autoimmune thyroid diseases.
We genotyped six single-nucleotide polymorphisms (SNPs), rs6865453 in selenoprotein P gene (SELENOP), rs713041 rs2074451 rs3746165 in glutathione peroxidase 4 gene (GPX4) and rs28665122 and rs7178239 in selenoprotein S gene (SELENOS) by MassARRAY system using the chip-based matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology in 1060 patients with autoimmune thyroid diseases and 938 healthy controls.
Major alleles in rs6865453 of SELENOP, rs713041, rs2074451, rs3746165 of GPX4 decreased while the major allele C in rs28665122 of SELENOS increased in AITD patients than in the control. The allele C and genotype CC in rs7178239 of SELENOS showed different trend in GD and HT patients when compared with the control. All the distribution difference showed nonsignificant. Analysis according to clinical features including ophthalmopathy, hypothyroidism and family history came out to be negative either.
Our findings suggest non-association between three selenoprotein genes and AITD, conflicting to the positive result in another population. Different selenium nutrition status in different populations may contribute to conflicting results, the contribution of genetic variants in AITD mechanism may be another reason.
We genotyped six single-nucleotide polymorphisms (SNPs), rs6865453 in selenoprotein P gene (SELENOP), rs713041 rs2074451 rs3746165 in glutathione peroxidase 4 gene (GPX4) and rs28665122 and rs7178239 in selenoprotein S gene (SELENOS) by MassARRAY system using the chip-based matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology in 1060 patients with autoimmune thyroid diseases and 938 healthy controls.
Major alleles in rs6865453 of SELENOP, rs713041, rs2074451, rs3746165 of GPX4 decreased while the major allele C in rs28665122 of SELENOS increased in AITD patients than in the control. The allele C and genotype CC in rs7178239 of SELENOS showed different trend in GD and HT patients when compared with the control. All the distribution difference showed nonsignificant. Analysis according to clinical features including ophthalmopathy, hypothyroidism and family history came out to be negative either.
Our findings suggest non-association between three selenoprotein genes and AITD, conflicting to the positive result in another population. Different selenium nutrition status in different populations may contribute to conflicting results, the contribution of genetic variants in AITD mechanism may be another reason.
摘要：
硒是必需的痕量，甲状腺中的硒浓度很高。硒缺乏可能损害甲状腺功能。本研究的目的是探讨三个硒蛋白基因多态性与自身免疫性甲状腺疾病之间的关系。
我们对六个单核苷酸多态性（SNPs）进行了基因分型，硒蛋白P基因(SELENOP)中的rs6865453,使用基于芯片的基质辅助激光解吸电离飞行时间MassARRAY系统，谷胱甘肽过氧化物酶4基因（GPX4）中的rs713041rs2074451rs3746165和硒蛋白S基因（SELENOS）中的rs28665122和rs7178239在1060名自身免疫性疾病患者和938名健康对照。
SELENOP的rs6865453中的主要等位基因，在AITD患者中，GPX4的rs713041，rs2074451，rs3746165降低，而SELENOS的rs28665122中的主要等位基因C升高。与对照组相比，GD和HT患者的SELENOSrs7178239中的等位基因C和基因型CC显示出不同的趋势。所有分布差异均不显著。根据包括眼病在内的临床特征进行分析，甲状腺功能减退症和家族史均为阴性。
我们的发现表明三个硒蛋白基因与AITD之间没有关联，与另一个群体的积极结果相冲突。不同人群中不同的硒营养状况可能导致相互矛盾的结果。遗传变异在AITD机制中的贡献可能是另一个原因。
我们对六个单核苷酸多态性（SNPs）进行了基因分型，硒蛋白P基因(SELENOP)中的rs6865453,使用基于芯片的基质辅助激光解吸电离飞行时间MassARRAY系统，谷胱甘肽过氧化物酶4基因（GPX4）中的rs713041rs2074451rs3746165和硒蛋白S基因（SELENOS）中的rs28665122和rs7178239在1060名自身免疫性疾病患者和938名健康对照。
SELENOP的rs6865453中的主要等位基因，在AITD患者中，GPX4的rs713041，rs2074451，rs3746165降低，而SELENOS的rs28665122中的主要等位基因C升高。与对照组相比，GD和HT患者的SELENOSrs7178239中的等位基因C和基因型CC显示出不同的趋势。所有分布差异均不显著。根据包括眼病在内的临床特征进行分析，甲状腺功能减退症和家族史均为阴性。
我们的发现表明三个硒蛋白基因与AITD之间没有关联，与另一个群体的积极结果相冲突。不同人群中不同的硒营养状况可能导致相互矛盾的结果。遗传变异在AITD机制中的贡献可能是另一个原因。
