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Abstract：
Kisspeptin, encoded by kiss1, has been regarded as a major modulator of mammalian puberty and fertility due to its stimulation on GnRH. Brandt\'s vole is one of the main pest species on the Inner Mongolian steppes for its striking reproductive capacity and kiss1 is a key candidate gene related to reproductive regulatory cascades. In this study, kiss1 cDNA was cloned from the hypothalamus of Brandt\'s voles and kiss1 mRNA levels were investigated in different tissues, and at different developmental stages, using high-throughput real-time PCR. The full-length kiss1 cDNA was 682bp, containing an ORF of 405bp, encoding 134 amino acids with a conserved kisspeptin-10 region. Kiss1 mRNA was specifically expressed in ovary, testicle, small intestine, kidney, liver and hypothalamus tissues, and was undetectable in other tissues, including pituitary, heart, adrenal gland, bladder and uterus. Sexual organs of both male and female voles enter a period of rapid development in the postnatal 4weeks and reach or approach sexual maturity by 8weeks after birth. Kiss1 mRNA levels in the hypothalamus did not show a significant difference between week 2 and week 4, indicating kiss1 mRNA levels may not be related to the rapid growth of the sexual organs in early developmental stages. Kiss1 transcripts significantly increased in both sexes 8weeks after birth, and then were maintained at high levels in adults, indicating its possible role in the onset of puberty and maintaining of reproductive activity. These results are helpful to further the study of kiss1 function in reproductive regulation of Brandt\'s voles.
摘要：
Kisspeptin,由kiss1编码，由于其对GnRH的刺激，被认为是哺乳动物青春期和生育能力的主要调节剂。勃兰特田鼠因其惊人的繁殖能力而成为内蒙古草原上的主要害虫之一，而kiss1是与生殖调节级联相关的关键候选基因。在这项研究中,从布兰特田鼠下丘脑克隆kiss1cDNA，并研究不同组织中kiss1mRNA水平，在不同的发展阶段，使用高通量实时PCR。kiss1的cDNA全长为682bp，含有405bp的ORF，编码134个氨基酸，具有保守的kisspeptin-10区域。Kiss1mRNA在卵巢中特异性表达，睾丸,小肠,肾,肝脏和下丘脑组织，在其他组织中检测不到，包括垂体,心,肾上腺,膀胱和子宫.雄性和雌性田鼠的性器官在出生后4周进入快速发育期，并在出生后8周达到或接近性成熟。下丘脑中的Kiss1mRNA水平在第2周和第4周之间没有显着差异，表明kiss1mRNA水平可能与早期发育阶段性器官的快速生长无关。出生后8周，两种性别的Kiss1转录本均显着增加，然后在成年人中保持在高水平，表明其在青春期开始和维持生殖活动中的可能作用。这些结果有助于进一步研究kiss1在Brandt田鼠生殖调节中的作用。
