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Abstract：
The maintenance of viable and functional pancreatic islets is crucial for successful islet transplantation from brain-dead donors. To overcome islet quality loss during culture, some studies have co-cultured islets with mesenchymal stem/stromal cells (MSC). However, it is still uncertain if MSC-secreted factors are enough to improve islet quality or if a physical contact between MSCs and islets is needed. Therefore, we performed a systematic review and meta-analysis to clarify the effect of different culture contact systems of islets with MSCs on viability and insulin secretion outcomes. Pubmed and Embase were searched. Twenty studies fulfilled the eligibility criteria and were included in the qualitative synthesis and/or meta-analysis. For both outcomes, pooled weighted mean differences (WMD) between islet cultured alone (control group) and the co-culture condition were calculated. Viability mean was higher in islets co-cultured with MSCs compared with islet cultured alone [WMD = 18.08 (95% CI 12.59-23.57)]. The improvement in viability was higher in islets co-cultured in indirect or mixed contact with MSCs than in direct physical contact (P <0.001). Moreover, the mean of insulin stimulation index (ISI) was higher in islets from co-culture condition compared with islet cultured alone [WMD = 0.83 (95% CI 0.54-1.13)], independently of contact system. Results from the studies that were analyzed only qualitatively are in accordance with meta-analysis data. Co-culture of islets with MSCs has the potential for protecting islets from injury during culture period. Moreover, culture time appears to influence the beneficial effect of different methods of co-culture on viability and function of islets.
摘要：
维持有活力和功能的胰岛对于从脑死亡供体成功移植胰岛至关重要。为了克服培养过程中的胰岛质量损失，一些研究已经将胰岛与间充质干细胞/基质细胞（MSC）共培养。然而,目前尚不确定MSC分泌的因子是否足以改善胰岛质量,或者是否需要MSC与胰岛进行身体接触.因此,我们进行了系统回顾和荟萃分析,以阐明胰岛与MSCs的不同培养物接触系统对活力和胰岛素分泌结局的影响.搜索Pubmed和Embase。20项研究符合资格标准，并纳入定性综合和/或荟萃分析。对于这两种结果，计算单独培养的胰岛(对照组)和共培养条件之间的合并加权平均差异(WMD).与单独培养的胰岛相比，与MSC共培养的胰岛的活力平均值更高[WMD=18.08（95％CI12.59-23.57）]。与MSC间接或混合接触共培养的胰岛的活力改善高于直接物理接触(P<0.001)。此外，与单独培养的胰岛相比，共培养条件下胰岛的胰岛素刺激指数（ISI）平均值更高[WMD=0.83（95％CI0.54-1.13）]，独立的接触系统。仅定性分析的研究结果与荟萃分析数据一致。胰岛与MSCs共培养具有在培养期间保护胰岛免受损伤的潜力。此外，培养时间似乎会影响不同共培养方法对胰岛活力和功能的有益作用。
