PDF(Sci-hub)
Abstract：
The magnitude of the current Zika virus (ZIKV) epidemic has led to a declaration of a Public Health Emergency of International Concern by the WHO. Findings of viable viral particles in semen for several weeks are corroborating reports of sexual transmission of ZIKV. Serious consequences of a positive diagnostic result particularly in the pregnant patient are calling for precise diagnostic tools also at later time points after infection. Currently, recommendations suggest a diagnostic period of direct viral detection of 5 to 7 days after onset of symptoms in serum or plasma, and up to 3 weeks in urine samples.
A vasectomized 41-year-old German returning from Martinique presented at the outpatient clinic of the Department for Infectious Diseases and Tropical Medicine, Munich, with subfebrile temperature, rash, malaise, severe retro-orbital pain and occipital lymphadenopathy. The main complaints resolved after ten days without specific treatment. We are reporting on clinical course and results of direct and indirect detection methods of ZIKV in different sample types including whole blood, ejaculate, urine, serum, plasma and saliva samples up to 119 days post symptom onset. Ejaculate samples remained PCR positive for ZIKV until day 77, whole blood samples until day 101.
The case presentation adds to the still limited knowledge of kinetics of detection of ZIKV by direct as well as indirect methods. Here, a complete data set including results from PCR, serology and cell culture is provided allowing an improved evaluation of optimum diagnostic periods for testing a variety of sample types. Moreover, a high viral load of ZIKV RNA was detected in ejaculate of the vasectomized patient. This finding sheds new light on the possible localizations of ZIKV replication in the human male reproductive tract.
A vasectomized 41-year-old German returning from Martinique presented at the outpatient clinic of the Department for Infectious Diseases and Tropical Medicine, Munich, with subfebrile temperature, rash, malaise, severe retro-orbital pain and occipital lymphadenopathy. The main complaints resolved after ten days without specific treatment. We are reporting on clinical course and results of direct and indirect detection methods of ZIKV in different sample types including whole blood, ejaculate, urine, serum, plasma and saliva samples up to 119 days post symptom onset. Ejaculate samples remained PCR positive for ZIKV until day 77, whole blood samples until day 101.
The case presentation adds to the still limited knowledge of kinetics of detection of ZIKV by direct as well as indirect methods. Here, a complete data set including results from PCR, serology and cell culture is provided allowing an improved evaluation of optimum diagnostic periods for testing a variety of sample types. Moreover, a high viral load of ZIKV RNA was detected in ejaculate of the vasectomized patient. This finding sheds new light on the possible localizations of ZIKV replication in the human male reproductive tract.
摘要：
当前寨卡病毒（ZIKV）流行的规模已导致世界卫生组织宣布了国际关注的突发公共卫生事件。精液中存活病毒颗粒数周的发现证实了ZIKV性传播的报道。阳性诊断结果的严重后果，特别是在怀孕患者中，在感染后的较晚时间点也需要精确的诊断工具。目前,建议在血清或血浆症状发作后5至7天进行直接病毒检测的诊断期，和长达3周的尿液样本。
一名从马提尼克岛返回的41岁接受输精管切除的德国人出现在传染病和热带医学部的门诊，慕尼黑,低热温度，皮疹,萎靡不振,严重的眶后疼痛和枕淋巴结病。主要投诉在没有具体治疗的十天后得到解决。我们正在报告不同样本类型（包括全血）中ZIKV的直接和间接检测方法的临床过程和结果，射精,尿液,血清,血浆和唾液样本长达119天症状发作后。射精样品对ZIKV保持PCR阳性直到第77天,全血样品直到第101天。
案例介绍增加了通过直接和间接方法检测ZIKV的动力学的仍然有限的知识。这里,包括PCR结果的完整数据集，本发明提供了血清学和细胞培养,其允许用于测试多种样品类型的最佳诊断期的改进评估。此外，在输精管切除患者的射精中检测到高病毒载量的ZIKVRNA.这一发现为ZIKV复制在人类男性生殖道中的可能定位提供了新的思路。
一名从马提尼克岛返回的41岁接受输精管切除的德国人出现在传染病和热带医学部的门诊，慕尼黑,低热温度，皮疹,萎靡不振,严重的眶后疼痛和枕淋巴结病。主要投诉在没有具体治疗的十天后得到解决。我们正在报告不同样本类型（包括全血）中ZIKV的直接和间接检测方法的临床过程和结果，射精,尿液,血清,血浆和唾液样本长达119天症状发作后。射精样品对ZIKV保持PCR阳性直到第77天,全血样品直到第101天。
案例介绍增加了通过直接和间接方法检测ZIKV的动力学的仍然有限的知识。这里,包括PCR结果的完整数据集，本发明提供了血清学和细胞培养,其允许用于测试多种样品类型的最佳诊断期的改进评估。此外，在输精管切除患者的射精中检测到高病毒载量的ZIKVRNA.这一发现为ZIKV复制在人类男性生殖道中的可能定位提供了新的思路。
