人 U6 小核 RNA 基因的体内转录可承受基因内序列的缺失，但不能承受上游 TATATA 框的缺失。Transcription of a human U6 small nuclear RNA gene in vivo withstands deletion of intragenic sequences but not of an upstream TATATA box.-医云文献数字医云科研云海量医学决策数据服务

Abstract：

Most eukaryotic genes transcribed by RNA polymerase III contain internal control regions. U6 small nuclear RNA genes are transcribed by RNA polymerase III but are unusual in that, at least in vitro, their expression does not require intragenic sequences. Here we show that this is true as well in vivo. A human U6 gene devoid of all but the first 6 and last 10 base-pairs was expressed efficiently after transfection into human 293 cells. We also report data extending the previous identification of 5\' flanking sequences important for human U6 gene transcription. Deletion-substitution of a 10 base-pair upstream sequence encompassing the TATATA element (-29 to -24) abolished U6 transcription. A double point mutation in the middle of this element (TATATA-TAGCTA) reduced U6 transcription but not to the extent brought about by TATATA deletion-substitution. These results establish that, in vivo, transcription of human U6 small nuclear RNA is independent of intragenic sequences between nucleotides 6 and 98, and requires the upstream TATATA box.

摘要：

暂无翻译

Transcription of a human U6 small nuclear RNA gene in vivo withstands deletion of intragenic sequences but not of an upstream TATATA box.

1 The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.

2 A distant enhancer element is required for polymerase III transcription of a U6 RNA gene.

3 A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.

4 A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.

5 The gene for the U6 small nuclear RNA in fission yeast has an intron.

6 Changing the RNA polymerase specificity of U snRNA gene promoters.

7 Upstream sequences modulate the internal promoter of the human 7SL RNA gene.

8 Rapid and efficient site-specific mutagenesis without phenotypic selection.

9 Surprises in polymerase III transcription.

10 Transcription of human 7S K DNA in vitro and in vivo is exclusively controlled by an upstream promoter.

11 Transcription of Neurospora crassa 5 S rRNA genes requires a TATA box and three internal elements.

12 Changing directions in Pol III transcription.

13 Rapid and efficient site-specific mutagenesis without phenotypic selection.

14 Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.

15 U6 small nuclear RNA is transcribed by RNA polymerase III.

16 OBP100 binds remarkably degenerate octamer motifs through specific interactions with flanking sequences.

17 Spliceosomal RNA U6 is remarkably conserved from yeast to mammals.

18 Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes.

19 Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.

20 A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.

21 The nucleotide sequence of nuclear U6 (4.7 S) RNA.

22 The capped U6 small nuclear RNA is transcribed by RNA polymerase III.

23 Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III.