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Abstract：
Simulated environmental colonisation of granite was induced under laboratory conditions in order to develop an experimental protocol for studying bioreceptivity. The experimental set-up proved suitable for producing subaerial biofilms by inoculating granite blocks with planktonic multi-species phototrophic cultures derived from natural biofilms. The ability of four different cultures to form biofilms was monitored over a three-month growth period via colour measurements, quantification of photosynthetic pigments and EPS, and CLSM observations. One of the cultures under study, which comprised several taxa including Bryophyta, Charophyta, Chlorophyta and Cyanobacteria, was particularly suitable as an inoculum, mainly because of its microbial richness, its rapid adaptability to the substratum and its high colonisation capacity. The use of this culture as an inoculum in the proposed experimental set-up to produce subaerial biofilms under laboratory conditions will contribute to standardising the protocols involved, thus enabling more objective assessment of the bioreceptivity of granite in further experiments.
摘要：
在实验室条件下诱导了花岗岩的模拟环境定殖，以开发用于研究生物容受性的实验方案。实验装置被证明适用于通过用天然生物膜衍生的浮游多物种光养培养物接种花岗岩块来生产地下生物膜。通过颜色测量在三个月的生长期内监测四种不同培养物形成生物膜的能力，光合色素和EPS的定量，和CLSM观测。正在研究的文化之一，其中包括几个类群，包括苔藓植物，Charophyta,绿藻和蓝细菌，特别适合作为接种物，主要是因为它的微生物丰富，它对基质的快速适应性和高定殖能力。在拟议的实验装置中使用这种培养物作为接种物，在实验室条件下生产地下生物膜将有助于标准化所涉及的方案。从而可以在进一步的实验中更客观地评估花岗岩的生物容受性。
