Abstract：
RNA nanoparticles are designed and self-assembled according to noncanonical interactions of naturally conserved RNA motifs and/or canonical Watson-Crick base-pairing interactions, which have potential applications in gene therapy and nanomedicine. These artificially engineered nanoparticles are mainly synthesized from in vitro transcribed RNAs, purified by denaturing and native polyacrylamide gel electrophoresis (PAGE), and characterized with native PAGE, AFM, and TEM technologies. The protocols of in vitro transcription, denaturing and native PAGE, and RNA nanoparticle self-assembly are described in detail.
摘要：
RNA纳米颗粒根据天然保守RNA基序的非规范相互作用和/或规范Watson-Crick碱基配对相互作用进行设计和自组装，在基因治疗和纳米医学中具有潜在的应用。这些人工工程化的纳米颗粒主要由体外转录的RNA合成，通过变性和天然聚丙烯酰胺凝胶电泳（PAGE）纯化，并以原生PAGE为特征，AFM,和TEM技术。体外转录的方案，变性和天然PAGE，和RNA纳米颗粒自组装的详细描述。
