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Abstract：
There is a significant correlation between the degree of tumor differentiation and the survival of patients with gastric cancers. In this report, we compared proteomic differences between poorly differentiated gastric adenocarcinoma tissues and well-differentiated gastric adenocarcinoma tissues in order to identify differentiation-related proteins that may be closely correlated with differentiation of gastric cancer pathogenesis. We identified 7 proteins, of which calreticulin precursor, tapasinERP57 heterodimer, pyruvate kinase isozymes M1/M2 isoform M2, class Pi glutathione S-transferase, and chain A crystal structure of human enolase 1 were upregulated in poorly differentiated gastric adenocarcinoma compared with well-differentiated gastric adenocarcinoma, while myosin-11 isoform SM2A and actin alpha cardiac were downregulated. Two of them, pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 are enzymes involved in glycolytic pathway. The upregulation of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 in poorly differentiated gastric adenocarcinoma was confirmed by Western blotting and immunohistochemistry. Furthermore, we observed 107 cases with gastric adenocarcinoma and found that the high expression of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 correlates with tumor size (P = .0001 and P = .0017, respectively), depth of invasion (P = .0024 and P = .0261, respectively), and poor prognosis of patients. In conclusion, with this proteomic analysis, pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 were identified upregulated in poorly differentiated gastric adenocarcinoma comparing with well-differentiated gastric adenocarcinoma. The expression level of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 was significantly correlated with overall survival. Some of them would be differentiation-related cancer biomarkers and are associated with tumor metastasis, invasion, and prognosis.
摘要：
肿瘤分化程度与胃癌患者的生存期有显著的相关性。在这份报告中,我们比较了低分化胃腺癌组织和高分化胃腺癌组织之间的蛋白质组差异,以鉴定可能与胃癌发病机制分化密切相关的分化相关蛋白.我们鉴定了7种蛋白质,其中钙网蛋白的前体，tapasinERP57异二聚体，丙酮酸激酶同工酶M1/M2亚型M2，Pi类谷胱甘肽S-转移酶，与高分化胃腺癌相比，低分化胃腺癌中人烯醇化酶1的链A晶体结构上调，而肌球蛋白11亚型SM2A和肌动蛋白α下调。其中两个,丙酮酸激酶同工酶M1/M2同种型M2和烯醇化酶1是参与糖酵解途径的酶。通过蛋白质印迹和免疫组织化学证实了低分化胃腺癌中丙酮酸激酶同工酶M1/M2亚型M2和烯醇化酶1的上调。此外，我们观察了107例胃腺癌，发现丙酮酸激酶同工酶M1/M2亚型M2和烯醇化酶1的高表达与肿瘤大小相关（分别为P=0.0001和P=0.0017），侵入深度（分别为P=.0024和P=.0261），患者预后不良。总之,通过这种蛋白质组学分析，与高分化胃腺癌相比，低分化胃腺癌中丙酮酸激酶同工酶M1/M2亚型M2和烯醇化酶1被上调。丙酮酸激酶同工酶M1/M2亚型M2和烯醇化酶1的表达水平与总生存期显着相关。其中一些是分化相关的癌症生物标志物，并且与肿瘤转移有关。入侵,和预后。
