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Abstract：
It has previously been shown that the use of racemic mixtures of naturally chiral macromolecules such as protein and DNA can significantly aid the crystallogenesis process, thereby addressing one of the major bottlenecks to structure determination by X-ray crystallographic methods-that of crystal growth. Although previous studies have provided convincing evidence of the applicability of the racemic crystallization technique to DNA through the study of well-characterized DNA structures, we sought to apply this method to a historically challenging DNA sequence. For this purpose we chose a non-self-complementary DNA duplex containing the biologically-relevant Pribnow box consensus sequence \'TATAAT\'. Four racemic crystal structures of this previously un-crystallizable DNA target are reported (with resolutions in the range of 1.65-2.3 Å), with further crystallographic studies and structural analysis providing insight into the racemic crystallization process as well as structural details of this highly pertinent DNA sequence.
摘要：
以前已经表明，使用天然手性大分子如蛋白质和DNA的外消旋混合物可以显着帮助晶体形成过程。从而解决了通过X射线晶体学方法确定结构的主要瓶颈之一-晶体生长。尽管先前的研究通过研究特征良好的DNA结构，提供了外消旋结晶技术对DNA适用性的令人信服的证据，我们试图将这种方法应用于具有历史挑战性的DNA序列。为此，我们选择了一种非自身互补的DNA双链体，其中包含生物学相关的Pribnowbox共有序列\“TATAAT”。报告了这种先前不可结晶的DNA靶标的四个外消旋晶体结构（分辨率在1.65-2.3的范围内），通过进一步的晶体学研究和结构分析，可以深入了解外消旋结晶过程以及这种高度相关的DNA序列的结构细节。
