抗生素抗肿瘤药物对培养肿瘤细胞中核苷酸水平的影响 : 基于靶向代谢组学区分抗肿瘤药物作用机制的探索性方法。
关键词: ADP, adenosine diphosphate AMP, adenosine monophosphate ANOVA, analysis of variance ATP, adenosine triphosphate AUC, area under the curve Antibiotic anticancer drugs CDP, cytidine diphosphate CTP, cytidine triphosphate DMEM, Dulbecco׳s modified eagle׳s cell culture media DMSO, dimethyl sulfoxide DNA, deoxyribonucleic acid EC, energy charge EDTA, ethylene diamine tetra-acetic acid FCS, fetal calf serum GDP, guanosine diphosphate GMP, guanosine monophosphate GTP, guanosine triphosphate HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid Ion-pair HPLC MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Mechanisms of antitumor drug action Nucleotides PBS, phosphate buffered saline PCA, principal component analysis Potential biomarkers Principal component analysis RNA, ribonucleic acid ROC, receiver operating characteristic RPMI-1640, Roswell Park Memorial Institute-1640 TBAHS, tetrabutylammonium hydrogen sulfate TCA, trichloroacetic acid Targeted metabolomics analysis Tumor cells UDP, uridine diphosphate UTP, uridine triphosphate dATP, deoxyadenosine triphosphate dCDP, deoxycytidine diphosphate dCTP, deoxycytidine triphosphate dGMP, deoxyribonucleic monophosphate dGTP, deoxyguanosine triphosphate dUDP, deoxyuridine diphpsphate dUTP, deoxyuridine triphosphate
来 源: DOI:10.1016/j.apsb.2015.03.010