Mesh : Chromosome Mapping Chromosomes, Plant Cotton Fiber Crosses, Genetic Gene Expression Genetic Linkage Genetic Markers Gossypium / genetics Microsatellite Repeats Phenotype Polymorphism, Single Nucleotide Quantitative Trait Loci Quantitative Trait, Heritable

来  源:   DOI:10.1371/journal.pone.0135430   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
Cotton (Gossypium hirsutum L.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. Technological developments in the textile industry and improvements in human living standards have increased the requirement for supplies and better quality cotton. Upland cotton 0-153 is an elite cultivar harboring strong fiber strength genes. To conduct quantitative trait locus (QTL) mapping for fiber quality in 0-153, we developed a population of 196 recombinant inbred lines (RILs) from a cross between 0-153 and sGK9708. The fiber quality traits in 11 environments were measured and a genetic linkage map of chromosome 25 comprising 210 loci was constructed using this RIL population, mainly using simple sequence repeat markers and single nucleotide polymorphism markers. QTLs were identified across diverse environments using the composite interval mapping method. A total of 37 QTLs for fiber quality traits were identified on chromosome 25, of which 17 were stably expressed in at least in two environments. A stable fiber strength QTL, qFS-chr25-4, which was detected in seven environments and was located in the marker interval between CRI-SNP120491 and BNL2572, could explain 6.53%-11.83% of the observed phenotypic variations. Meta-analysis also confirmed the above QTLs with previous reports. Application of these QTLs could contribute to improving fiber quality and provide information for marker-assisted selection.
摘要:
棉花(GossypiumhirsutumL.)是一种重要的农作物,为全球纺织工业提供了可再生的天然纤维资源。纺织工业的技术发展和人类生活水平的提高增加了对供应和质量更好的棉花的需求。陆地棉0-153是具有强纤维强度基因的优良品种。为了对0-153中的纤维质量进行数量性状基因座(QTL)作图,我们从0-153和sGK9708之间的杂交中开发了196个重组自交系(RIL)。测量了11种环境中的纤维品质性状,并使用该RIL群体构建了包含210个基因座的25号染色体遗传连锁图谱,主要使用简单序列重复标记和单核苷酸多态性标记。使用复合间隔作图方法在不同环境中识别QTL。在25号染色体上共鉴定出37个纤维品质性状的QTL,其中17个在至少两种环境中稳定表达。一个稳定的纤维强度QTL,qFS-chr25-4在七个环境中检测到,位于CRI-SNP120491和BNL2572之间的标记区间,可以解释6.53%-11.83%的观察到的表型变异。Meta分析也证实了上述QTL与以前的报道。这些QTL的应用可以有助于改善纤维质量并为标记辅助选择提供信息。
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