关键词: APC, Allophycocyanin BCG, Mycobacterium bovis bacille Calmette-Guérin BSA, bovine serum album CMI, cell-mediated immune responses DAPI, 4′,6-diamidino-2-phenylindole DMSO, Dimethyl sulfoxide ELISPOT, Enzyme-linked immune-sorbent spot FACS, Fluorescence Activated Cell Sorter FBS, fetal bovine serum FITC, fluorescein isothiocyanate HA tag, hemagglutinin tag HEK, human embryo kidney ICS, Intracellular cytokine staining IFN-γ, interferon gamma IL-2, Interleukin 2 IM, intramuscular IN, intranasal Mtb, Mycobacterium tuberculosis NBT/BCIP, Nitro blue tetrazolium/ 5-Bromo-4-chloro-3-indolyl phosphate PBS, Phosphate Buffered Saline PCR, polymerase chain reaction PE, Phycoerythrin PerCP, Peridinin-ChlorophylL-Protein Complex RPMI, Roswell Park Memorial Institute SC, subcutaneous SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis SFC, spot-forming cells TB, tuberculosis TNF-α, tumor necrosis factor α fusion strategies immunization routes immunogenicity multiple antigens mycobacterium tuberculosis rAd5, recombinant adenovirus type 5 tPA, tissue plasminogen activator vp, viral particles
Mesh : Adenoviridae / genetics Administration, Oral Animals BCG Vaccine / immunology CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Cytokines / metabolism Female Genetic Vectors Immunity, Cellular Lung / immunology Mice Mice, Inbred C57BL Spleen / immunology Tuberculosis Vaccines / administration & dosage genetics immunology Vaccines, Synthetic / administration & dosage genetics immunology
来 源: DOI:10.1080/21645515.2015.1042193