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Abstract：
Obesity which developes due to multifactorial reasons, was associated recently with human Adenovirus-36 (Ad-36). The aim of this study was to investigate the prevalence of Ad-36 antibodies in obese adults and also to investigate the DNA of Ad-36 in their adipose tissue. In this cross-sectional and case-control based study, 49 obese adults, with BMI ≥ 30 kg/m(2), and 49 non-obese adults, with BMI ≤ 25 kg/m(2), applied for esthetic purposes and were included in this study as patient and control groups, respectively. Adipose tissue samples, obtained by the lipoaspiration method, were studied by single-step PCR and nested-PCR methods. Simultaneously, the presence of Ad-36 antibodies and serum leptin and adiponectin levels were assessed by serum neutralization assay (SNA) and ELISA, respectively. Serum samples which didn\'t cause a cytopathic effect at ≥ 1:8 were accepted as positive. Ad-36 antibody was detected in 6 (12.2%) of 49 patients by SNA and was statistically significant (p < 0.05). Ad-36 DNA was not detected in any of the adipose tissue samples of the patient or control groups. Mean BMI and leptin levels were higher in the Ad-36-positive group, while adiponectin levels were found to be lower in the Ad-36-positive group. Although no statistically significant difference was found in cholesterol and triglyceride levels between the two groups (p > 0.05), lower mean serum cholesterol and triglyceride levels were found in the Ad-36-positive patients. In conclusion, we couldn\'t detect Ad-36 DNA in adipose tissue; however, we detected significantly higher Ad-36 antibody levels in the obese group compared to the non-obese group, according to the both univariant and multivariant analyses, suggesting that Ad-36 may play a role in obesity. There is a need for new and extended serial, particularly cohort and human-based, studies in order to have a clear understanding of the Ad-36-obesity relationship.
摘要：
由于多因素原因而发展的肥胖，最近与人腺病毒36（Ad-36）有关。这项研究的目的是调查肥胖成年人中Ad-36抗体的患病率，并研究其脂肪组织中Ad-36的DNA。在这项基于横断面和病例对照的研究中，49名肥胖成年人，BMI≥30kg/m(2)，和49名非肥胖成年人，BMI≤25kg/m(2)，用于美学目的，并作为患者和对照组纳入本研究，分别。脂肪组织样本,通过脂肪抽吸方法获得，通过单步PCR和巢式PCR方法进行了研究。同时,通过血清中和测定（SNA）和ELISA评估Ad-36抗体的存在以及血清瘦素和脂联素水平，分别。在≥1:8时没有引起细胞病变效应的血清样本被接受为阳性。通过SNA在49例患者中的6例（12.2%）中检测到Ad-36抗体，具有统计学意义（p<0.05）。在患者或对照组的任何脂肪组织样品中均未检测到Ad-36DNA。Ad-36阳性组的平均BMI和瘦素水平较高，而Ad-36阳性组的脂联素水平较低。两组间胆固醇和甘油三酯水平差异无统计学意义(p>0.05),Ad-36阳性患者的平均血清胆固醇和甘油三酯水平较低。总之,我们无法在脂肪组织中检测到Ad-36DNA；然而，我们检测到肥胖组的Ad-36抗体水平显著高于非肥胖组,根据单变量和多变量分析，这表明Ad-36可能在肥胖中起作用。需要新的和扩展的串行，特别是队列和基于人类的，为了清楚了解Ad-36与肥胖的关系。
