关键词: 5, 5′-tetramethylbenzidine; BSA, bovine serum albumin CDR, complementarity-determining region ELISA, enzyme-linked immunosorbent assay HRP, horseradish peroxidase IS, internal standard IgG1, immunoglobulin G1 LC-MS/MS LC-MS/MS, liquid chromatography tandem mass spectrometry LDL-c, low density lipoprotein cholesterol LDLR, low density lipoprotein receptor LLOQ, lower limit of quantification MAD, multiple-ascending dose MQC, minimum quantifiable concentration MRM, multiple reaction monitoring NHS, normal human sera PBS, phosphate buffered saline PCSK9, proprotein convertase subtilisin/kexin type 9; PD, pharmacodynamics PK, pharmacokinetics RG7652 RT, room temperature S/N, signal-to-noise SA, streptavidin SAD, single-ascending dose SIL, stable isotope-labeled TMB, 3, 3′ clinical pharmacokinetic assay enzyme-linked immunosorbent assay mAbs, monoclonal antibodies proprotein convertase subtilisin/kexin type 9 rhuPCSK9, recombinant human PCSK9 signature peptide
Mesh : Antibodies, Monoclonal / blood immunology pharmacokinetics Antibodies, Monoclonal, Humanized Chromatography, Liquid Cohort Studies Dose-Response Relationship, Drug Double-Blind Method Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G / blood immunology Proprotein Convertase 9 Proprotein Convertases / immunology Serine Endopeptidases / immunology Tandem Mass Spectrometry