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Abstract：
Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm(2) , 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation. Immunofluorescent expression of cytokeratin 10 (red) and Cyclin D1 (green) in (A) Control keratinocytes and (B) Low-level laser irradiated cells. Blue color illustrates the nuclei of the cells (DAPI staining).
摘要：
低水平激光治疗（LLLT）已被广泛用于改善上皮伤口愈合，尽管LLLT后上皮成熟和分层的确切反应尚不清楚。因此，这项研究旨在评估用LLLT治疗时角质形成细胞（KCs）的体外生长和分化以及体内伤口愈合反应。人KC（HaCaT细胞）在所有使用的激光能量密度（3、6和12J/cm（2），660nm,100mW),以及细胞周期蛋白D1的表达增加。此外，与上皮增殖和成熟相关的蛋白质（p63，CK10，CK14）的免疫表达均表明LLLT治疗的伤口中迁移的KC成熟更快。这样,LLLT利用所采用的参数促进了上皮愈合的改善;与上皮增殖和成熟相关的几种蛋白质表达的变化证实了这种改善。细胞角蛋白10(红色)和细胞周期蛋白D1(绿色)在(A)对照角质形成细胞和(B)低水平激光照射细胞中的免疫荧光表达。蓝色表示细胞的细胞核(DAPI染色)。
