Abstract：
Dl-3n-butylphthalide can effectively treat cerebral ischemia; however, the mechanisms underlying the effects of dl-3n-butylphthalide on microcirculation disorders following diffuse brain injury remain unclear. In this study, models of diffuse brain injury were established in Sprague-Dawley rats with the vertical impact method. Dl-3n-butylphthalide at 80 and 160 mg/kg was given via intraperitoneal injection immediately after diffuse brain injury. Ultrastructural changes in the cerebral cortex were observed using electron microscopy. Cerebral blood flow was measured by laser Doppler flowmetry, vascular density was marked by tannic acid-ferric chloride staining, vascular permeability was es-timated by the Evans blue method, brain water content was measured using the dry-wet method, and rat behavior was measured by motor function and sensory function tests. At 6, 24, 48, and 72 hours after administration of dl-3n-butylphthalide, reduced cerebral ultrastructure damage, creased vascular density and cerebral blood flow, and improved motor and sensory functions were observed. Our findings demonstrate that dl-3n-butylphthalide may have protective effects against diffuse brain injury by ameliorating microcirculation disorder and reducing blood-brain barrier age and cerebral edema.
摘要：
丁苯酞可有效治疗脑缺血;dl-3丁苯酞对弥漫性脑损伤后微循环障碍的作用机制尚不清楚.在这项研究中,采用垂直冲击法建立大鼠弥漫性脑损伤模型。弥漫性脑损伤后立即通过腹膜内注射给予80和160mg/kg的D1-3正丁基苯酞。使用电子显微镜观察大脑皮层的超微结构变化。通过激光多普勒血流仪测量脑血流量，血管密度通过单宁酸-氯化铁染色标记，血管通透性由伊文思蓝方法估计，脑含水量采用干湿法测定，通过运动功能和感觉功能测试来测量大鼠的行为。在给予dl-3n-丁基苯酞后6、24、48和72小时，减少脑超微结构损伤，血管密度和脑血流量增加，并观察到运动和感觉功能的改善。我们的发现表明，dl-3n-丁基苯酞可能通过改善微循环障碍，减少血脑屏障年龄和脑水肿而对弥漫性脑损伤具有保护作用。
