METHODS: Pooled subgingival biofilm samples were obtained from 40 patients with chronic periodontitis and 40 healthy controls. Taqman q-PCR assays were used to determine the absolute and relative counts of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Parvimonas micra, Filifactor alocis, oral Synergistetes and oral TM7s. Microbial associations were assessed using cluster analysis. Different statistical models were used to explore associations between microbial parameters and periodontitis.
RESULTS: The median log and relative counts were lowest for TM7s (4.4 and 0.0016%, respectively) and highest for oral Synergistetes (7.2 and 1.4%, respectively). Oral Synergistetes clustered strongly with the red complex, particularly T. forsythia (100% rescaled similarity). All species/phylotypes except TM7s were significantly associated with periodontitis (Mann-Whitney test; p ≤ 0.005). However, P. gingivalis and F. alocis lost association after adjusting for confounders (ordinal regression). In receiving operator characteristic curve analysis, the log counts of oral Synergistetes were the best markers of periodontitis (82.5% sensitivity and specificity), followed by those of T. forsythia, P. micra and T. denticola. In prediction analysis, however, P. micra was the only microbial predictor of periodontal parameters.
CONCLUSIONS: Oral Synergistetes are presented here as new members of the red complex, with relative importance to periodontitis exceeding that of the classical members. P. micra is shown as an important periodontal pathogen warranting more attention.
方法:收集40例慢性牙周炎患者和40例健康对照者的龈下生物膜样本。Taqmanq-PCR测定用于确定牙龈卟啉单胞菌的绝对和相对计数,连翘坦菌,Denticola密螺旋体,Parvimonasmicra,Filifactoralocis,口服协同作用和口服TM7。使用聚类分析评估微生物关联。使用不同的统计模型来探索微生物参数与牙周炎之间的关联。
结果:TM7的对数中位数和相对计数最低(4.4和0.0016%,分别)和口服协同剂最高(7.2%和1.4%,分别)。口腔协同剂与红色复合物强烈聚集,特别是连翘毛虫(100%重新缩放相似性)。除TM7外,所有物种/基因型均与牙周炎显着相关(Mann-Whitney检验;p≤0.005)。然而,牙龈卟啉单胞菌和F.alocis在校正混杂因素后失去关联(序数回归)。在接受操作员特性曲线分析时,口腔协同剂的对数计数是牙周炎的最佳标志物(82.5%的敏感性和特异性),其次是连翘的,P.micra和T.denticola。在预测分析中,然而,P.micra是牙周参数的唯一微生物预测因子。
结论:口腔协同作用是红色复合体的新成员,对牙周炎的相对重要性超过经典成员。P.micra被证明是一种重要的牙周病原体,值得更多的关注。