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Abstract：
Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.
摘要：
血清学试验被广泛接受用于诊断弓形虫，但抗原的纯化和标准化需要改进。最近,为此，表面速殖子和缓生子抗原变得更具吸引力。在这项研究中,对3种重组抗原（SAG1、SAG2和SAG3）的诊断价值进行了评估，并将其功效与可用的商业ELISA进行了比较。将重组质粒转化到大肠杆菌JM109菌株中,表达和纯化重组体。在ELISA系统中使用不同的血清组评估重组SAG1，SAG2和SAG3抗原，并将结果与市售IgG和IgMELISA试剂盒的结果进行比较。与市售ELISA相比，重组表面抗原检测抗弓形虫IgG的敏感性和特异性如下：SAG1（93.6％和92.9％），SAG2（100.0%和89.4%），和SAG3（95.4%和91.2%），分别。在检测IgG抗弓形虫抗体方面,在重组SAG2和商业ELISA之间观察到高度一致(96.9%)。与其他2种重组抗原相比，P22在检测抗弓形虫IgM方面具有最佳性能。重组SAG1、SAG2和SAG3均可用于诊断针对弓形虫的IgG特异性抗体。
