关键词: Barcoding bats phylogeny

Mesh : Animals Chiroptera / genetics DNA, Mitochondrial / genetics Evolution, Molecular Genome, Mitochondrial Phylogeny Quality Control Sequence Analysis, DNA

来  源:   DOI:10.3109/19401736.2014.900666

Abstract:
Mitogenomic data are increasingly used in evolutionary biology and ecology, stressing the importance for double checking the authenticity of DNA sequences. For example, Szcześniak et al. (2013) recently published the mitochondrial genome of a bat, the Leschenault\'s rousette (Rousettus leschenaultii). Here we show using straightforward phylogenetic analyses of available chiropteran sequence data that the taxonomic attribution of the reported mitogenome is erroneous. The purportedly-new complete mitochondrial genome likely belongs to the Egyptian fruit bat (R. aegyptiacus) for which a reference sequence already exists. We propose that future articles reporting complete mitochondrial genome sequences should mandatorily include maximum likelihood trees inferred from (i) the standard barcoding marker for the taxon under focus, which would benefit from the massive data available in public databases, and (ii) the available mitogenomes of closely related species. We also strongly advise these trees be presented as phylograms so that all pertinent phylogenetic information is displayed in the form of a topology and its associated branch lengths. Along with compulsory information on the geographical location and origin of the specimen, these new standards should help avoiding the publication of taxonomically misidentified mitogenomes that might end up as reference sequences in public databases and re-used in subsequent meta-analyses.
摘要:
有丝分裂基因组数据越来越多地用于进化生物学和生态学,强调仔细检查DNA序列真实性的重要性。例如,Szcze_niak等人。(2013)最近发表了蝙蝠的线粒体基因组,Leschenault\'srousette(Rousettusleschenaultii)。在这里,我们使用可用的翼翅目序列数据的直接系统发育分析显示,所报道的有丝分裂基因组的分类学归属是错误的。据称新的完整线粒体基因组可能属于埃及果蝙蝠(R。埃及伊蚊),其参考序列已经存在。我们建议报告完整线粒体基因组序列的未来文章应强制包括从(i)重点分类单元的标准条形码标记推断的最大似然树,这将受益于公共数据库中可用的大量数据,和(ii)密切相关物种的可用有丝分裂基因组。我们还强烈建议将这些树显示为系统发育图,以便以拓扑结构及其相关分支长度的形式显示所有相关的系统发育信息。连同有关标本地理位置和来源的强制性信息,这些新标准应有助于避免发表分类学错误识别的有丝分裂原,这些有丝分裂原可能最终作为参考序列出现在公共数据库中,并在随后的荟萃分析中重复使用.
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