Abstract：
Recent studies have demonstrated that cathepsin K seems to be a powerful marker in identifying the microphthalmia associated transcription factor (MITF) family tumors such as renal perivascular epithelioid cell neoplasms (PEComas), alveolar soft part sarcoma, and translocation-associated renal cell carcinomas. However, the expression of cathepsin K in melanocytic lesions has not been well characterized. Our aim was to investigate the expression of cathepsin K in a wide histological spectrum of melanocytic lesions and to evaluate its potential diagnostic and molecular target therapy usefulness in comparison with other commonly used markers. 143 consecutive melanocytic lesions were selected for study including 56 primary malignant melanomas, 62 metastatic melanomas, and 25 benign nevi (16 intradermal melanocytic nevi and 9 compound melanocytic nevi). 107 of the 118 (91%) primary and metastatic melanomas displayed a high percentage of cells with moderately to strongly positive reactions for cathepsin K (mean 82%; range 0-95%). MITF, HMB45, Melan-A, and S100 were expressed in 85, 76, 78 and 96% of cases, respectively, with various percentages of positive cells (mean, 63, 49, 55 and 86%; range 0-90, 0-80, 0-90 and 0-95%). Among the benign nevi, cathepsin K, MITF, HMB45, Melan-A, and S100 were expressed in 88, 80, 36, 68 and 100% of cases, respectively. Cathepsin K appears to be consistently and strongly expressed in melanocytic lesions and valuable in distinguishing malignant melanomas from the majority of human cancers.
摘要：
最近的研究表明，组织蛋白酶K似乎是识别小眼症相关转录因子（MITF）家族肿瘤如肾血管周围上皮样细胞肿瘤（PEComas）的有力标记，肺泡软组织肉瘤，和易位相关的肾细胞癌。然而,组织蛋白酶K在黑素细胞病变中的表达尚未得到很好的表征。我们的目的是研究组织蛋白酶K在黑素细胞病变的广泛组织学谱中的表达，并与其他常用标志物进行比较，评估其潜在的诊断和分子靶向治疗的有效性。选择143个连续的黑素细胞病变进行研究，包括56个原发性恶性黑色素瘤，62个转移性黑素瘤，良性痣25例(皮内黑素细胞痣16例,复合黑素细胞痣9例)。118例（91％）原发性和转移性黑色素瘤中的107例显示出高百分比的细胞对组织蛋白酶K具有中度至强阳性反应（平均82％；范围0-95％）。MITF,HMB45,Melan-A,S100在85、76、78和96％的病例中表达，分别,具有不同百分比的阳性细胞(平均值，63、49、55和86%；范围为0-90、0-80、0-90和0-95%）。在良性痣中，组织蛋白酶K,MITF,HMB45,Melan-A,和S100在88、80、36、68和100%的病例中表达，分别。组织蛋白酶K似乎在黑素细胞病变中始终且强烈表达，并且在区分恶性黑素瘤与大多数人类癌症方面具有价值。
