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Abstract：
BACKGROUND: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations.
METHODS: We added (125)I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting.
RESULTS: Adsorption of urine albumin (UA) at 5-6 mg/l was <0.9%; and at 90 mg/l was <0.4%. Adsorption was generally less at pH8 than pH5 but only 3 cases had p<0.05. Adsorption from 11 unaltered urine samples with albumin 5-333 mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100 ml and 2l collection containers, and to instrument sample cups and showed <1% change in concentration at 5 mg/l and <0.5% change at 20 mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine.
CONCLUSIONS: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.
METHODS: We added (125)I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting.
RESULTS: Adsorption of urine albumin (UA) at 5-6 mg/l was <0.9%; and at 90 mg/l was <0.4%. Adsorption was generally less at pH8 than pH5 but only 3 cases had p<0.05. Adsorption from 11 unaltered urine samples with albumin 5-333 mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100 ml and 2l collection containers, and to instrument sample cups and showed <1% change in concentration at 5 mg/l and <0.5% change at 20 mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine.
CONCLUSIONS: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators.
摘要：
背景：白蛋白吸附到尿液收集和分析容器上可能会导致浓度过低。
方法：我们将（125）I标记的人血清白蛋白添加到尿液和磷酸盐缓冲溶液中，将它们与22个塑料容器材料一起孵育，并通过液体闪烁计数来测量吸附。
结果：在5-6mg/l时，尿白蛋白（UA）的吸附率<0.9％；在90mg/l时<0.4％。在pH8时的吸附通常小于pH5,但只有3例具有p<0.05。用白蛋白5-333mg/l从11个未改变的尿液样品中吸附<0.8%。将具有最大结合的材料的白蛋白吸附外推至100毫升和2升收集容器的表面积，和仪器样品杯，并显示<1％的变化在5毫克/升和<0.5％的变化在20毫克/升或更高的浓度。磷酸盐缓冲溶液中白蛋白的吸附量（2-28％）大于尿液中白蛋白的吸附量。
结论：白蛋白吸附在尿样和塑料材料之间存在差异，但是对于所有测试的材料和尿液样品，吸附的总影响<1％。白蛋白从磷酸盐缓冲溶液中的吸附量大于从尿液中的吸附量，这可能是用作校准品的制剂的限制。
方法：我们将（125）I标记的人血清白蛋白添加到尿液和磷酸盐缓冲溶液中，将它们与22个塑料容器材料一起孵育，并通过液体闪烁计数来测量吸附。
结果：在5-6mg/l时，尿白蛋白（UA）的吸附率<0.9％；在90mg/l时<0.4％。在pH8时的吸附通常小于pH5,但只有3例具有p<0.05。用白蛋白5-333mg/l从11个未改变的尿液样品中吸附<0.8%。将具有最大结合的材料的白蛋白吸附外推至100毫升和2升收集容器的表面积，和仪器样品杯，并显示<1％的变化在5毫克/升和<0.5％的变化在20毫克/升或更高的浓度。磷酸盐缓冲溶液中白蛋白的吸附量（2-28％）大于尿液中白蛋白的吸附量。
结论：白蛋白吸附在尿样和塑料材料之间存在差异，但是对于所有测试的材料和尿液样品，吸附的总影响<1％。白蛋白从磷酸盐缓冲溶液中的吸附量大于从尿液中的吸附量，这可能是用作校准品的制剂的限制。
