关键词： 3′ UTR 3′ untranslated regions AIDS Acquired Immune Deficiency Syndrome C/EBPα CCAAT/enhancer-binding protein α CTLs DAVID Deep-sequencing Differentially expression EBV ER ERAP Epstein–Barr virus FBS HCMV HCMV IL-10 HEL HSV-1 Human cytomegalovirus Human embryonic lung IE KEGG KSHV Kaposi's sarcoma-associated herpesvirus Kyoto encyclopedia of Genes and Genomes LATs LAcmvIL-10 LUNA Latent infection MAPKs MICB MOI MiRNA RT-PCR TBE THP-1 The Database for Annotation, Visualization and Integrated Discovery UCSC University of California, Santa Cruz cytotoxic T lymphocytes endoplasmic reticulum endoplasmic reticulum associated aminopeptidase fetal bovine serum herpes simplex virus 1 human cytomegalovirus human monocytic leukemia cell line immediate early latency unique nuclear antigen latency-associated cmvIL-10 latency-associated transcripts mRNAs mTOR major histocompatibility complex class 1-related chain B mammalian target of rapamycin messenger RNAs miRNAs microRNAs mitogen-activated protein kinase multiplicity of infection nt nucleotides qPCR quantitative real-time PCR reverse transcriptase PCR smRNA small RNA small nuclear RNA snRNA tris–borate-EDTA vIL-10

Mesh ： Cell Line Cell Line, Tumor Cytomegalovirus / genetics Cytomegalovirus Infections / genetics virology Gene Expression / genetics Humans Leukemia, Myeloid / genetics virology MicroRNAs / genetics Transcriptome / genetics Virus Latency / genetics

来  源：   DOI：10.1016/j.gene.2013.12.012   PDF(Sci-hub)

Abstract：
BACKGROUND: MicroRNAs (miRNAs) play important roles in regulating gene expression of plants, animals and viruses. Comprehensive characterization of host and viral miRNA will help uncover the molecular mechanisms that underlie the progression of human cytomegalovirus (HCMV) latent infection. To investigate the miRNA expression profile of HCMV and host cells during latent infection, we performed deep-sequencing analysis of the small RNAs isolated from HCMV-infected and mock-infected human monocytic leukemia cell line, THP-1.
RESULTS: We established a HCMV latent infection cell model using the THP-1 cells. High-throughput sequencing technology was used to sequence small RNA libraries of the HCMV-infected and mock-infected THP-1 and to investigate their small RNA transcriptomes. We found eight miRNAs including miR-US25-1, miR-US25-2-5p and miR-UL112 that were expressed by HCMV during latent infection. The expressions of the host miRNAs were also affected by HCMV latent infection. At least 49 cellular miRNAs were differentially expressed: 39 were up-regulated and 10 were down-regulated upon HCMV latent infection. The expression of the human miRNA hsa-miR-124-3p was significantly up-regulated in the HCMV latent infection library. In addition, we found 14 cellular novel miRNAs in the HCMV-infected and mock-infected THP-1 libraries. Functional annotation of the target genes of the differentially expressed miRNAs suggested that the majority of the genes are involved in melanogenesis, pathways in cancer, endocytosis and wnt signaling pathway.
CONCLUSIONS: The small RNA transcriptomes obtained in this study demonstrate the usefulness of the deep-sequencing combined with bioinformatics approach in understanding of the expression and function of host and viral small RNAs in HCMV latent infection. This approach can also be applied to the study of other kinds of viruses.
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