Polysaccharides with immunomodulating properties from the bark of Parkia biglobosa.

• 影响指数: 10.723
• 发表时间: Jan 2014 30
• 来源期刊: Carbohydr Polym
• DOI: 10.1016/j.carbpol.2013.09.082
• PMID: 24299798
• 文章类型: Journal Article
• 中科院分区: Q1;Q1;Q1
• 作者列表: Zou YF,Zhang BZ,Inngjerdingen KT,Barsett H,Diallo D,Michaelsen TE,El-Zoubair E,Paulsen BS

关键词: 4-O-methyl-glucuronic acid; 4-OMe-GlcA; AG-I; AG-II; Ara; Complement system; GC–MS; Gal; GalA; Glc; GlcA; Immunomodulation; LPS; Macrophage stimulation; Man; NO; Parkia biglobosa; Pectic polysaccharides; RG-I; RG-II; Rha; Xyl; arabinogalactan type I; arabinogalactan type II; arabinose; galactose; galacturonic acid; gas chromatography–mass spectrometry; glucose; glucuronic acid; lipopolysaccharide; mannose; nitric oxide; rhamnogalacturonan type I; rhamnogalacturonan type II; rhamnose; xylose

Mesh: Animals; Cell Line; Ethanol / chemistry; Fabaceae / chemistry; Immunologic Factors / chemistry isolation & purification pharmacology; Macrophages / drug effects immunology metabolism; Mice; Molecular Weight; Nitric Oxide / metabolism; Phenol / analysis; Plant Bark / chemistry; Plant Proteins / analysis; Polysaccharides / chemistry isolation & purification pharmacology; Temperature; Water / chemistry

Abstract

The bark of Parkia biglobosa is used in traditional medicine to cure a wide range of illnesses. Polysaccharides were extracted from the bark with 50% ethanol-water, 50°C and 100°C water, and seven active fractions obtained by anion exchange chromatography and gel filtration. The complement fixation and macrophage stimulating activities of the different fractions were determined. The acidic fractions PBEII-I and PBEII-IV were the most active in the complement fixation assay, but the other fractions were also potent compared to the positive control BPII from Biophytum petersianum. Fractions PBEII-I and PBEII-IV were also the most potent fractions in stimulating macrophages to release nitric oxide. Structural studies showed that PBEII-I and PBEII-IV were pectic type polysaccharides, containing arabinogalactan type II structures. The observed differences in biological activities among the seven purified polysaccharide sub-fractions are probably due to differences in monosaccharide compositions, linkage types and molecular sizes.