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Abstract：
Aquaporins belongs to the major intrinsic proteins involved in the transcellular membrane transport of water and other small solutes. A comprehensive genome-wide search for the homologues of Solanum tuberosum major intrinsic protein (MIP) revealed 41 full-length potato aquaporin genes. All potato aquaporins are grouped into five subfamilies; plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and x-intrinsic proteins (XIPs). Functional predictions based on the aromatic/arginine (ar/R) selectivity filters and Froger\'s positions showed a remarkable difference in substrate transport specificity among subfamilies. The expression pattern of potato aquaporins, examined by qPCR analysis, showed distinct expression profiles in various organs and tuber developmental stages. Furthermore, qPCR analysis of potato plantlets, subjected to various abiotic stresses revealed the marked effect of stresses on expression levels of aquaporins. Taken together, the expression profiles of aquaporins imply that aquaporins play important roles in plant growth and development, in addition to maintaining water homeostasis in response to environmental stresses.
摘要：
水通道蛋白属于参与水和其他小溶质的跨细胞膜运输的主要内在蛋白。对马铃薯主要内在蛋白（MIP）同源物的全面全基因组搜索揭示了41个全长马铃薯水通道蛋白基因。所有马铃薯水通道蛋白分为五个亚家族;质膜固有蛋白(PIPs),液泡膜内在蛋白（TIPs），NOD26样内在蛋白（NIPs），小的碱性内在蛋白(SIPs)和X-内在蛋白(XIPs)。基于芳香族/精氨酸（ar/R）选择性过滤器和Froger的位置的功能预测显示，亚家族之间的底物转运特异性存在显着差异。马铃薯水通道蛋白的表达模式,通过qPCR分析检查，在各个器官和块茎发育阶段显示出不同的表达谱。此外，马铃薯试管苗的qPCR分析，受到各种非生物胁迫，表明胁迫对水通道蛋白表达水平的显着影响。一起来看,水通道蛋白的表达谱表明水通道蛋白在植物生长发育中起着重要作用,除了维持水的稳态以应对环境压力。
