关键词: amniotic membrane transplantation child eosinophil shield ulcer vernal keratoconjunctivitis

Mesh : Adolescent Amnion / transplantation Child Conjunctivitis, Allergic / metabolism pathology surgery Cornea / surgery Eosinophil Major Basic Protein / analysis Humans Immunohistochemistry Male

来  源:   DOI:10.1016/j.jfma.2012.07.017   PDF(Sci-hub)

Abstract:
Two children with shield ulcer in vernal keratoconjunctivitis unresponsive to steroid therapy received plaque removal by superficial keratectomy, followed by amniotic membrane transplantation (AMT). Hematoxylin and eosin staining of the excised corneal specimen revealed a thick layer of eosinophilic material attached to the Bowman\'s layer. These deposits were positive for eosinophil granule major basic protein, as confirmed by an immunohistochemical study. The shield ulcer healed after the amniotic membrane was removed. No recurrent corneal plaque developed, although corneal opacity complicated in both cases. Lamellar keratectomy with AMT offers an effective management by removing the cytotoxic plaques and protecting the denuded stroma from deposition of inflammatory debris.
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