大鼠和小鼠背根神经节神经元和外周轴突 CaV3.2 T 型电压门控钙通道表达的免疫组织学证明。
关键词: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 4’, 6-diamidino-2-phenylindole CGRP CNS Ca(2+) DAPI DRG DTNB EM GA GFP HEK HEPES IB(4) KI KO MYE Mas-related G protein-coupled receptor D Mrgprd NF200 NONMYE PB PBS PFA T-channels T-currents T-type calcium channels TBST Tris buffer with 0.9% NaCl and Triton-X-100 WT calcitonin-gene-related-peptide central nervous system di-thio nitro benzene dorsal root ganglion electron microscopy glutaraldehyde green fluorescent protein human embryonic kidney isolectin B4 knock-in knock-out low-voltage-activated myelinated neurofilament 200 nociceptors paraformaldehyde phosphate buffer phosphate-buffered saline unmyelinated wild type
Mesh : Animals Antibodies Axons / metabolism Calcium Channels, T-Type / biosynthesis genetics immunology Cells, Cultured Ganglia, Spinal / metabolism Humans Immunohistochemistry Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Electron Nerve Fibers / drug effects metabolism physiology ultrastructure Neurons / metabolism Nociceptors / drug effects physiology Rats Rats, Sprague-Dawley Sciatic Nerve / cytology metabolism Skin / metabolism
来 源:
DOI:10.1016/j.neuroscience.2013.07.005
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