Identification and genetic effect of haplotype in the bovine BMP7 gene.

• 影响指数: 3.913
• 发表时间: Dec 2013 15
• 来源期刊: Gene
• DOI: 10.1016/j.gene.2013.03.009
• PMID: 23500594
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 生物
• 作者列表: Huang YZ,Wang XL,He H,Lan XY,Lei CZ,Zhang CL,Chen H

关键词: Association; BMP7; BMPs; Bone morphogenetic proteins; CH; Cattle; Chinese Holstein; GLM; Growth traits; HWE; Haplotype; Hardy–Weinberg equilibrium; He; JX; Jiaxian; LD; LSM; NY; Nanyang; Ne; PAGE; PCR–SSCP; PIC; QC; Qinchuan; SNPs; SPSS; TBE; TGF-β; UCP1; base pair(s); bp; effective allele numbers; general linear models; heterozygosity; is a buffer solution containing a mixture of Tris base, boric acid and EDTA; least square means; linkage disequilibrium; polyacrylamide gel electrophoresis; polymerase chain reaction–single stranded conformational polymorphism; polymorphism information content; single nucleotide polymorphisms; statistical product and service solutions; transforming growth factor-beta; uncoupling protein 1

Mesh: Animals; Body Size / genetics; Body Weight / genetics; Bone Morphogenetic Protein 7 / genetics; Cattle / genetics growth & development; Gene Frequency; Genetic Association Studies; Haplotypes; Heart / anatomy & histology growth & development; Linkage Disequilibrium; Organ Size / genetics; Polymorphism, Single Nucleotide; Sequence Analysis, DNA

Abstract

Bone morphogenetic proteins (BMPs) are peptide growth factors belonging to the transforming growth factor-beta (TGF-β) superfamily, and some members of the BMP family support white adipocyte differentiation. In this study, we focused on the BMP7 which singularly promotes the differentiation of brown preadipocytes. Haplotypes involving 5 single nucleotide polymorphism (SNP) sites in the bovine BMP7 gene were identified and their effect on body weight was analyzed. 16 haplotypes and 18 combined haplotypes were revealed and the linkage disequilibrium was assessed in the cattle population with 602 individuals representing three main cattle breeds from China. The results showed that haplotypes 3, 10 and 14 were predominant and accounted for 75.64%, 69.85%, and 83.36% in Nanyang, Qinchuan and Jiaxian cattle breeds, respectively. The statistical analyses indicated that the SNP 1, 4, and 5 are associated with the body weight, body length, and heart girth at 12 and 24 months in Nanyang cattle population (P<0.05), whereas there is no significant association between their 16 haplotypes and 18 combined haplotypes. Our results provide evidence that some SNPs and haplotypes in BMP7 are associated with growth traits, and may be utilized as a genetic marker in marker-assisted selection for beef cattle breeding programs.