关键词: Chlamydomonas reinhardtii Chlorophyll Z PS II QA YD YD-less mutant YZ positions pulsed EPR

来  源:   DOI:10.1023/A:1020312621949   PDF(Sci-hub)

Abstract:
PELDOR (Pulsed Electron eLectron DOuble Resonance) was applied to determinethe distance of between Y(Z)and Q(A) (-)inY(D)-less mutant of Chlamydomonas reinhardtiiin Tris-treatedand Zn-substituted preparation of photosystem II. The value of distance wasfound to be 34.5 ± 1 Â. A \'2+1\' electron spin echo method has beenapplied to measure the orientation of the radius-vector RfomY(D)to Chl(Z)in a membrane-oriented photosystem II. The anglebetween Rand the membrane normal nwas determined to be 50 ±5(°), using the distance 29.4 ± 0.5 Â determined in non-orientedPS II.
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