Mesh : Directed Molecular Evolution / methods High-Throughput Screening Assays / methods Mutagenesis, Site-Directed / methods Mutant Proteins / genetics Protein Engineering / methods

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Abstract:
As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering.
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