{Reference Type}: English Abstract {Title}: [Design and application of high-throughput screening tools: a review]. {Author}: Tang S;Liang C;Jiang P; {Journal}: Sheng Wu Gong Cheng Xue Bao {Volume}: 28 {Issue}: 7 {Year}: Jul 2012 暂无{DOI}: {Abstract}: As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering.