Abstract：
Antigenic drift is the ability of the swine influenza virus to undergo continuous and progressive changes in response to the host immune system. These changes dictate influenza vaccine updates annually to ensure inclusion of antigens of the most current strains. The identification of those peptides that stimulate T-cell responses, termed T-cell epitopes, is essential for the development of successful vaccines. In this study, the highly conserved and specific epitopes from neuraminidase of globally distributed H1N1 strains were predicted so that these potential vaccine candidates may escape with antigenic drift. A total of nine novel CD8(+) T-cell epitopes for MHC class-I and eight novel CD4(+) T-cell epitopes for MHC class-II alleles were proposed as novel epitope based vaccine candidates. Additionally, the epitope FSYKYGNGV was identified as a highly conserved, immunogenic and potential vaccine candidate, capable for generating both CD8(+) and CD4(+) responses.
摘要：
抗原性漂移是猪流感病毒响应宿主免疫系统而经历连续和进行性变化的能力。这些变化要求每年更新流感疫苗，以确保包含最新毒株的抗原。那些刺激T细胞反应的肽的鉴定，称为T细胞表位，对于开发成功的疫苗至关重要。在这项研究中,预测了全球分布的H1N1毒株的神经氨酸酶的高度保守和特异性表位，因此这些潜在的疫苗候选物可能因抗原漂移而逃脱。提出了总共9个用于I类MHC的新型CD8（）T细胞表位和8个用于II类MHC等位基因的新型CD4（）T细胞表位作为基于表位的新型疫苗候选物。此外,表位FSYKYGNGV被鉴定为高度保守的，免疫原性和潜在的疫苗候选，能够产生CD8（+）和CD4（+）反应。
