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Abstract：
Filamentous bacteriophages (filamentous bacterial viruses or Inovirus) are simple and well-characterised macromolecular assemblies that are widely used in molecular biology and biophysics, both as paradigms for studying basic biological questions and as practical tools in areas as diverse as immunology and solid-state physics. The strains fd, M13 and f1 are virtually identical filamentous phages that infect bacteria expressing F-pili, and are sometimes grouped as the Ff phages. For historical reasons fd has often been used for structural studies, but M13 and f1 are more often used for biological experiments. Many other strains have been identified that are genetically quite distinct from Ff and yet have a similar molecular structure and life cycle. One of these, Pf1, gives the highest resolution X-ray fibre diffraction patterns known for filamentous bacteriophage. These diffraction patterns have been used in the past to derive a molecular model for the structure of the phage. Solid-state NMR experiments have been used in separate studies to derive a significantly different model of Pf1. Here we combine previously published X-ray fibre diffraction data and solid-state NMR data to give a consensus structure model for Pf1 filamentous bacteriophage, and we discuss the implications of this model for assembly of the phage at the bacterial membrane.
摘要：
丝状噬菌体（丝状细菌病毒或Inovirus）是简单且特征明确的大分子组装体，广泛用于分子生物学和生物物理学。既作为研究基本生物学问题的范式，又作为免疫学和固体物理学等不同领域的实用工具。菌株fd，M13和f1是几乎相同的丝状噬菌体，感染表达F-菌毛的细菌，有时被分组为Ff噬菌体。由于历史原因，FD经常被用于结构研究，但是M13和f1更常用于生物实验。已经鉴定了许多其他菌株,它们在遗传上与Ff完全不同,但仍具有相似的分子结构和生命周期。其中一个,Pf1给出了已知用于丝状噬菌体的最高分辨率X射线纤维衍射图。过去已经使用这些衍射图案来推导噬菌体结构的分子模型。固态NMR实验已用于单独的研究中，以得出明显不同的Pf1模型。在这里，我们结合以前发表的X射线纤维衍射数据和固态NMR数据，给出了Pf1丝状噬菌体的一致结构模型，我们讨论了该模型对噬菌体在细菌膜上组装的影响。
