Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides: case study on engineered apidaecins.

• 影响指数: 3.322
• 发表时间: Apr 2010 23
• 来源期刊: Biochem Biophys Res Commun
• DOI: 10.1016/j.bbrc.2010.03.088
• PMID: 20302844
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Matsumoto K,Orikasa Y,Ichinohe K,Hashimoto S,Ooi T,Taguchi S

Mesh: Amino Acid Sequence; Antimicrobial Cationic Peptides / genetics metabolism pharmacology; Escherichia coli / drug effects metabolism; Flow Cytometry / methods; Fluoresceins / chemistry metabolism; Fluorescent Dyes / chemistry metabolism; Molecular Sequence Data; Protein Engineering; Protein Transport

Abstract

Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency.