Pressure as a tool to study protein-unfolding/refolding processes: the case of ribonuclease A.

压力作为研究蛋白质解折叠 / 重折叠过程的工具 ： 核糖核酸酶 A 的情况

• 发表时间: Mar 2006
• 来源期刊: Biochim Biophys Acta
• DOI: 10.1016/j.bbapap.2005.11.011
• PMID: 16388998
• 文章类型: Journal Article
• 作者列表: Ribó M,Font J,Benito A,Torrent J,Lange R,Vilanova M

Mesh: Animals; Humans; Pressure; Protein Conformation; Protein Folding; Ribonuclease, Pancreatic / chemistry genetics

Abstract

This paper gives an overview of the application of high-pressure to study the folding/unfolding processes of proteins using Ribonuclease A as a model protein. A particular focus is the study of pressure-equilibrium unfolding and folding kinetics using variants and the information obtained by comparing these with the wild-type enzyme.

摘要

本文综述了以核糖核酸酶A为模型蛋白质，应用高压研究蛋白质的折叠/解折叠过程。特别关注的是使用变体的压力平衡解折叠和折叠动力学的研究以及通过将其与野生型酶进行比较而获得的信息。