Abstract:
The population genetic composition of Poa annua L. was studied by starch electrophoresis along a transect running NE from an organic reagents factory at Shanghai, China. Five enzyme systems were stained. We have reached the following preliminary conclusions: (1). Organic pollution has dramatically changed genotypic frequencies at some loci of Poa annua populations. At polluted sites, significant deviations from Hardy-Weinberg equilibrium were observed on loci Sod-1 and Me due to the excess of heterozygote. Especially in the two nearest sites to pollution source, all the individuals were heterozygous at locus Sod-1. The data suggests that heterozygotes were more tolerant to organic pollution than homozygotes, indicating the fitness superiority of heterozygotes. (2). A tendency towards clinal changes of allele frequencies was found at some polymorphic loci. Frequencies of the common alleles at loci Sod-1, Me and Fe-1 increased as the distance to the pollution source increased. (3). The effective number of alleles per locus, and the observed and expected heterozygosity were much higher in the pollution series than in the clear control site (Botanic Park population), but genetic multiplicity (number of alleles per locus) was lower than for the control. (4). Most genetic variability was found within populations, and only 2.56% were among populations of the polluted series. However, 9.48% of the total genetic variation occurred among populations when including the Botanic Park population. The genetic identity between populations of the pollution series (0.9869-1.0000, mean 0.9941) was higher than those between the pollution series and the Botanic Park population. UPGMA divided the five populations into two groups. One contained the four polluted populations, and the other only contained the Botanic Park population.
摘要:
通过淀粉电泳沿着上海一家有机试剂厂的NE运行的样带研究了PoaAnnuaL.的种群遗传组成,中国。5个酶系统被染色。我们得出了以下初步结论:(1)。有机污染已极大地改变了早熟禾种群某些基因座的基因型频率。在污染的地方,由于杂合子过多,在Sod-1和Me基因座上观察到与Hardy-Weinberg平衡的显着偏差。特别是在离污染源最近的两个地点,所有个体在Sod-1位点均为杂合。数据表明,杂合子比纯合子对有机污染的耐受性更高,表明杂合子的适应度优势。(2).在某些多态位点发现了等位基因频率发生基因变化的趋势。随着与污染源的距离增加,Sod-1,Me和Fe-1基因座上常见等位基因的频率增加。(3).每个基因座的有效等位基因数,并且在污染系列中观察到的和预期的杂合性比在明确的控制区(植物园种群)中高得多,但是遗传多重性(每个基因座的等位基因数)低于对照。(4).大多数遗传变异是在种群内发现的,污染系列人群中只有2.56%。然而,当包括植物园种群时,总遗传变异的9.48%发生在种群之间。污染系列种群之间的遗传同一性(0.9869-1.0000,平均0.9941)高于污染系列与植物园种群之间的遗传同一性。UPGMA将五个种群分为两组。其中一个包含四个受污染的人群,另一个只包含植物园的人口。