Mesh : Algorithms Brain / metabolism Cell Line DNA, Complementary / metabolism Humans Polymerase Chain Reaction / methods Reverse Transcriptase Polymerase Chain Reaction Time Factors

来  源:   DOI:10.1006/meth.2001.1262   PDF(Sci-hub)

Abstract:
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
摘要:
暂无翻译
公众号