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Abstract：
Similarity between the DNA substrates and products of integrase-mediated site-specific recombination reactions results in a single recombinase enzyme being able to catalyze both the integration and excision reactions. The control of directionality in these reactions is achieved through a class of small accessory factors that favor one reaction while interfering with the other. These proteins, which we will refer to collectively as recombination directionality factors (RDFs), play architectural roles in reactions catalyzed by their cognate recombinases and have been identified in conjunction with both tyrosine and serine integrases. Previously identified RDFs are typically small, basic and have diverse amino acid sequences. A subset of RDFs, the cox genes, also function as transcriptional regulators. We present here a compilation of all the known RDF proteins as well as those identified through database mining that we predict to be involved in conferring recombination directionality. Analysis of this group of proteins shows that they can be grouped into distinct sub-groups based on their sequence similarities and that they are likely to have arisen from several independent evolutionary lineages. This compilation will prove useful in recognizing new proteins that confer directionality upon site-specific recombination reactions encoded by plasmids, transposons, phages and prophages.
摘要：
DNA底物和整合酶介导的位点特异性重组反应的产物之间的相似性导致单一重组酶能够催化整合和切除反应。这些反应中的方向性的控制是通过一类有利于一个反应而干扰另一个反应的小辅助因素来实现的。这些蛋白质，我们将其统称为重组方向性因子(RDF)，在由其同源重组酶催化的反应中发挥结构作用，并且已与酪氨酸和丝氨酸整合酶一起被鉴定。以前识别的RDF通常很小，具有不同的氨基酸序列。RDF的子集，cox基因,也作为转录调节因子。我们在这里提供了所有已知的RDF蛋白质以及通过数据库挖掘鉴定的蛋白质的汇编，我们预测这些蛋白质参与赋予重组方向性。这组蛋白质的分析表明，它们可以根据它们的序列相似性分为不同的亚组，并且它们可能来自几个独立的进化谱系。该汇编将被证明有助于识别新的蛋白质，这些蛋白质赋予质粒编码的位点特异性重组反应的方向性，转座子,噬菌体和预言。
