目的:研究重组α-丝芬(LUF)的抗癌特性。然而,将LUF封装在Niosomes内对SKBR3乳腺癌细胞系的细胞毒性作用尚待研究.我们的研究旨在研究这种包封策略是否可以改善细胞毒性作用。
方法:Alpha-liffin表达,纯化,重新折叠。然后,这种蛋白质被用来制作最佳配方,以实验设计为指导。在这项工作中,我们探索了各种物理化学性质,包括颗粒大小,多分散指数,zeta电位,形态学,截留效率,药物释放和动力学,储存稳定性,和FTIR光谱。此外,我们评估了优化的脂质体制剂对SKBR3乳腺癌细胞系的细胞摄取和细胞毒性作用.
结果:优化的脂质体表现出315±6.4nm(DLS)的平均直径。成功地将LUF封装成规则形状,实现了球形囊体,封装效率为73.45±2.4%。值得注意的是,小脂质体LUF(NLUF)对SKBR3细胞表现出显著增加的细胞毒性。
结论:这些研究结果表明,负载LUF的niosome有望成为乳腺癌的潜在治疗策略。
OBJECTIVE: The anticancer properties of recombinant α-luffin (LUF) are wellestablished. However, the cytotoxic effects of encapsulating LUF within niosomes on the SKBR3 breast cancer cell line have yet to be explored. Our study aimed to investigate whether this encapsulation strategy could improve cytotoxic effects.
METHODS: Alpha-luffin was expressed, purified, and refolded. Then, this protein was utilized to craft an optimal formulation, guided by experimental design. In this work, we have explored various physicochemical properties, including particle size, polydispersity index, zeta potential, morphology, entrapment efficiency, drug release and kinetics, storage stability, and FTIR spectroscopy. Additionally, we have assessed the cellular uptake and cytotoxic effect of the optimized niosome formulation on the SKBR3 breast cancer cell line.
RESULTS: The optimized niosome exhibited a mean diameter of 315±6.4 nm (DLS). Successful encapsulation of LUF into regularly shaped, spherical niosomes was achieved, with an encapsulation efficiency of 73.45±2.4%. Notably, Niosomal LUF (NLUF) exhibited significantly increased cytotoxicity against SKBR3 cells.
CONCLUSIONS: These findings suggest that niosomes loaded with LUF hold promise as a potential treatment strategy for breast cancer.