背景:在足球中,大多数研究评估男性运动员的代谢特征变化,经常使用来自单个匹配的数据。鉴于目前的景观女子足球和生物性别对生理反应和运动适应的影响,更多针对女运动员的研究,并分析整个赛季的赛前和赛后时刻是必要的。
目的:描述来自巴西精英团队的女足球运动员的代谢组学概况。该研究观察了赛前和赛后三个时刻的群体分离,并确定了有区别的代谢物。
方法:这项研究包括14名女子足球运动员。在三场全国冠军赛的赛前和赛后时刻,使用核磁共振收集并分析了尿液样本。然后将代谢组学数据用于产生OPLS-DA和VIP图。
结果:在样品中鉴定出43种代谢物。OPLS-DA分析证明了前后条件之间的逐步分离,由增加的Q2值(游戏1、2和3分别为0.534、0.625和0.899)和第一分量值(游戏1和2中的20.2%和19.1%与游戏3中的29.9%)。15种最具鉴别力的代谢物中有8种在三场比赛中一致出现:甘氨酸,甲酸盐,柠檬酸盐3-羟基戊酸,乙醇酸,三甲胺,尿素,还有二甲基甘氨酸.
结论:三场比赛之间的主要区别是在整个锦标赛中各组之间的间隔越来越大。由于VIP分数较高的代谢物与能量和蛋白质代谢有关,这种分离可以归因于几个因素,一个是疲劳的积累。
BACKGROUND: In soccer, most studies evaluate metabolic profile changes in male athletes, often using data from a single match. Given the current landscape of women\'s soccer and the effects of biological sex on the physiological response and adaptation to exercise, more studies targeting female athletes and analyzing pre- and post-game moments throughout the season are necessary.
OBJECTIVE: To describe the metabolomics profile of female soccer athletes from an elite team in Brazil. The study observed the separation of groups in three pre- and post-game moments and identified the discriminating metabolites.
METHODS: The study included 14 female soccer athletes. Urine samples were collected and analyzed using Nuclear Magnetic Resonance in pre-game and immediate post-game moments over three national championship games. The metabolomics data were then used to generate OPLS-DA and VIP plots.
RESULTS: Forty-three metabolites were identified in the samples. OPLS-DA analyses demonstrated a progressive separation between pre-post conditions, as supported by an increasing Q2 value (0.534, 0.625, and 0.899 for games 1, 2 and 3, respectively) and the first component value (20.2% and 19.1% in games 1 and 2 vs. 29.9% in game 3). Eight out of the fifteen most discriminating metabolites appeared consistently across the three games: glycine, formate, citrate, 3-hydroxyvalerate, glycolic acid, trimethylamine, urea, and dimethylglycine.
CONCLUSIONS: The main difference between the three games was the increasing separation between groups throughout the championship. Since the higher VIP-scores metabolites are linked to energy and protein metabolism, this separation may be attributed several factors, one being the accumulation of fatigue.