背景:扁平苔藓(LP)是一种病因不明的慢性炎症性皮肤病。LP免疫发病机制主要由介导基底角质形成细胞免疫应答的细胞毒性T淋巴细胞控制。它可能转化为能够引发自身免疫反应的抗原库。然而,其他致病途径补充这些机制。最近的研究强调亚硝化应激参与慢性炎症性皮肤病的发病机制。关于其在LP发病机理中的作用的当前数据很少。
方法:在本文中,我们调查了40例皮肤LP(CLP)患者的硝化应激与40例健康受试者相比,使用血清标志物包括硝化应激标志物-直接亚硝酸盐,总亚硝酸盐,硝酸盐和对称二甲基精氨酸(SDMA),总抗氧化剂状态(TAS),和hsCRP,炎症的标志,并分析了亚硝化胁迫之间的关系,抗氧化防御,和炎症为NO途径在LP发病机制中的作用提供了新的见解。
结果:我们发现直接亚硝酸盐的血清水平明显升高,总亚硝酸盐,硝酸盐,SDMA和hsCRP,与对照组相比,CLP患者的TAS水平显着降低。CLP患者血清TAS水平之间存在显着负相关,血清hsCRP水平与分析的硝化应激标志物之间存在显着正相关。
结论:我们的结果表明LP患者亚硝基应激水平升高,这与促炎状态和抗氧化防御改变有关。
BACKGROUND: Lichen planus (LP) is a chronic inflammatory skin disease of unelucidated etiology. LP immunopathogenesis is mainly governed by cytotoxic T lymphocytes that mediate an immune response in basal keratinocytes, which may transform into a reservoir of antigens able to initiate an autoimmune reaction. However, other pathogenic pathways complement these mechanisms. Recent studies highlight the involvement of nitrosative stress in the pathogenesis of chronic inflammatory skin diseases. Current data on its role in the pathogenesis of LP are scarce.
METHODS: In this article, we investigated nitrosative stress in 40 cutaneous LP (CLP) patients compared to 40 healthy subjects using serum markers including nitrosative stress markers-direct nitrite, total nitrite, nitrate and symmetric dimethylarginine (SDMA), total antioxidant status (TAS), and hsCRP, a marker of inflammation, and analyzed the relationship between nitrosative stress, antioxidant defense, and inflammation to offer new insights into the role of the NO pathway in LP pathogenesis.
RESULTS: We identified significantly higher serum levels of direct nitrite, total nitrite, nitrate, SDMA and hsCRP, and significantly lower levels of TAS in CLP patients versus controls. There were significant negative correlations between the serum levels of TAS and significantl positive correlations between the serum levels of hsCRP and the analyzed nitrosative stress markers in patients with CLP.
CONCLUSIONS: Our results indicate an increased level of nitrosative stress in LP patients that correlates with a pro-inflammatory status and altered antioxidant defense.