Litoria(L.)paraewingi(沃森等人,1971)(大河州立森林,Victoria)是第一次在这里描述。它是在脚趾修剪浸渍剂的组织培养后制备的,冷冻保存,再培养和常规4',6-二氨基-2-苯基吲哚(DAPI)染色。然后将L.paraewingi核型与类似处理的IUCN(国际自然保护联盟)最不关心的成员L.ewingii(DumériletBibron,1841年)(维多利亚州南部)和叶尔维西斯(DumerriletBibron,1841年)(迈尔湖国家公园,新南威尔士州),同一个L.ewingii建筑群/组的所有成员。L.paraewingi二倍体数为2n=26,与其他两个物种相同。Litoriaparaewingi染色体1、2、6和7是亚中心的,染色体3和5是亚中心的,其余的是亚中心的。在任何评分的L.paraewingi中期传播中,常规DAPI染色后,均未发现继发性收缩或推定的核仁组织区域(NOR)。相反,在其他两个物种的所有检查中期传播中,在1号染色体的长臂上很容易识别出推定的NOR。这里的L.ewingii和L.jervisiensis的核型与L.paraewingi进一步不同,对于两个以前的物种,1号染色体是亚中心的,8号和10号染色体是亚中心的。在DAPI染色方面,叶尔维西尼核型与艾文吉和帕拉温吉的核型不同,其特征是:(i)亚中心3号染色体和亚中心4号染色体的明显相对长度倒置,以及(ii)6号染色体是亚中心而不是亚中心。所有三个物种相对于染色体2、5、7、9、11、12和13具有高度保守的染色体形态。在核型上观察到最大的总体形态差异。这些核型数据支持先前基于遗传相容性和行为的这三个物种的系统发育分离,生化和分子遗传分析。
The
karyotype of Litoria (L.) paraewingi (Watson et al., 1971) (Big River State Forest, Victoria) is described here for the first time. It is prepared following tissue culture of toe clipping macerates, cryopreservation, reculture and conventional 4\',6-diamidino-2-phenylindole (DAPI) staining. The L.paraewingi
karyotype is then compared to similarly processed IUCN (International Union for the Conservation of Nature) least concern members L.ewingii (Duméril et Bibron, 1841) (southern Victoria) and L.jervisiensis (Duméril et Bibron, 1841) (Myall Lakes National Park, New South Wales), all members of the same L.ewingii complex/group. The L.paraewingi diploid number is 2n = 26, the same as for the other two species. Litoriaparaewingi chromosomes 1, 2, 6 and 7 are submetacentric, chromosomes 3 and 5 are subtelocentric and the remainder are metacentric. No secondary constriction or putative nucleolus organiser region (NOR) was readily identifiable following conventional DAPI staining in any scored L.paraewingi metaphase spread. Conversely, a putative NOR was readily identifiable on the long arm of chromosome 1 in all examined metaphase spreads for the other two species. The karyotypes of L.ewingii and L.jervisiensis here further differ from L.paraewingi with chromosome 1 being metacentric and chromosomes 8 and 10 being submetacentric for both former species. The L.jervisiensis
karyotype differs from those of L.ewingii and L.paraewingi by DAPI staining with: (i) apparent relative length inversion of subtelocentric chromosome 3 and metacentric chromosome 4 and (ii) chromosome 6 being metacentric rather than submetacentric. All three species have a highly conserved chromosome morphology with respect to chromosomes 2, 5, 7, 9, 11, 12 and 13. The greatest gross morphological difference karyotypically is observed between L.paraewingi and L.jervisiensis. These
karyotype data support the previous phylogenetic separation of these three species based upon genetic compatibility and behavioural, biochemical and molecular genetic analyses.