■建立了离心超滤-高效液相色谱-串联质谱(HPLC-MS/MS)方法,以测定癫痫儿童中的游离perampanel(PER)浓度。
■使用离心超滤装置获得游离PER浓度。内标为PER-D5。研究了该方法的选择性,结转,定量下限,校正曲线,准确度,精度,矩阵效应,recovery,和稳定性。Spearman相关系数用于评估游离和总PER浓度之间的相关性。使用非参数检验来评估PER和其他抗癫痫药物对总和游离PER浓度的影响。
■57个血浆样品中游离PER浓度与总PER浓度呈正相关(r=0.793>0,P<0.001)。此外,丙戊酸(VPA)联合治疗组(9.87±5.83)的游离PER浓度显著高于非VPA联合治疗组(5.03±4.57)。
■所提出的方法是有效的,敏感,适用于癫痫患儿的游离PER浓度检测。同时,癫痫患儿的游离PER浓度对临床结局的反应更具临床意义,特别是与VPA结合使用时。
UNASSIGNED: The centrifugal ultrafiltration-high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to determine the free perampanel (PER) concentration in children with epilepsy.
UNASSIGNED: Free PER concentration was obtained using centrifugal ultrafiltration devices. The internal standard was PER-D5. The method was investigated for selectivity, carryover, lower limit of quantification, calibration curve, accuracy, precision, matrix effects, recovery, and stability. The Spearman\'s correlation coefficient was used to evaluate the correlation between the free and total PER concentrations. A nonparametric test was used to estimate the effects of PER along with other antiepileptic drugs on the total and free PER concentrations.
UNASSIGNED: The free PER concentration was positively correlated with the total PER concentration in the 57 plasma samples (r = 0.793 > 0, P < 0.001). Additionally, the free PER concentrations were significantly (P < 0.05) increased in valproic acid (VPA) co-therapy (9.87 ± 5.83) compared with non-VPA co-therapy (5.03 ± 4.57).
UNASSIGNED: The proposed method is efficient, sensitive, and suitable for detecting free PER concentrations in children with epilepsy. Simultaneously, the free PER concentration response to clinical outcomes in children with epilepsy was more clinically significant, particularly when combined with VPA.