鸡肉加工会产生大量的副产品,未充分利用或处置不当。在这项研究中,我们采用计算机方法鉴定鸡肝副产物中的抗氧化肽。值得注意的是,肽WYR表现出显着的2,2-偶氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除活性,IC50为0.13±0.01mg/mL,并在各种条件下表现出稳定性。包括热,pH值,NaCl,和模拟胃肠消化。分子对接分析揭示了显著的氢键相互作用,而分子动力学显示出与ABTS和2,2-二苯基-1-吡啶酰肼(DPPH)的不同稳定性。WYR表现出改善的抗应力性,活性氧(ROS)水平降低,提高了超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,并通过胰岛素/胰岛素样生长因子(IIS)信号通路调节关键基因的表达,丝裂原活化蛋白激酶(MAPK),和热休克转录因子-1(HSF-1)途径。这些作用共同促进了秀丽隐杆线虫寿命的延长。这项研究不仅为抗氧化肽分析提供了一种有效的方法,而且还强调了提高家禽副产品利用率的潜力。
Chicken meat processing generates a substantial number of byproducts, which are either underutilized or improperly disposed. In this study, we employed in silico approaches to identify antioxidant peptides in chicken liver byproducts. Notably, the peptide WYR exhibited remarkable 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity with an IC50 of 0.13 ± 0.01 mg/mL and demonstrated stability under various conditions, including thermal, pH, NaCl, and simulated gastrointestinal digestion. Molecular docking analysis revealed significant hydrogen bonding interactions, while molecular dynamics showed differential stability with ABTS and 2,2-Diphenyl-1-picrylhydrazyl (DPPH). WYR exhibited improved stress resistance, decreased levels of reactive oxygen species (ROS), elevated the activities of superoxide dismutase (SOD) and catalase (CAT), and modulated the expression of crucial genes through the insulin/insulin-like growth factor (IIS) signaling pathway, mitogen-activated protein kinase (MAPK), and heat shock transcription factor-1 (HSF-1) pathways. These effects collectively contributed to the extension of Caenorhabditis elegans\' lifespan. This study not only provides an effective method for antioxidant peptide analysis but also highlights the potential for enhancing the utilization of poultry byproducts.