孤独症中的线粒体功能障碍导致线粒体合成三磷酸腺苷(ATP)的能力受到柠檬酸循环的损害,并增加无氧糖酵解。目的-测量和评估线粒体标记的水平;包括谷氨酸草酰乙酸转氨酶(GOT),谷氨酸丙酮酸转氨酶(GPT),苹果酸脱氢酶,和丙酮酸激酶)在自闭症组中,并且知道使用这些标志物诊断自闭症谱系障碍儿童的可能性。在Al-Zahraa教学医院(库特市,伊拉克)对100名伊拉克儿童(男女),之间(2023年4月至2024年1月)。他们的年龄在3到9岁之间。其中50例患者作为孤独症组,50例健康者作为对照组。收集血样并对GOT进行生物测定,GPT,丙酮酸激酶,用ELISA技术测定苹果酸脱氢酶。自闭症组显示尿液有,尿液GPT,血清苹果酸,ASD组血清丙酮酸水平明显高于对照组(P<0.001)。ROC分析显示尿液中,尿液中,血清苹果酸和血清丙酮酸的准确度为(81%,71%,77%,和80%),曲线下面积(AUC)>0.7(0.8),0.7、0.7(0.76)、和0.7(0.8)因此尿液,尿液GPT,血清,苹果酸,血清丙酮酸是有效的诊断标记物。线粒体标志物的平均尿液和血清浓度存在显着差异(GOT,GPT,苹果酸脱氢酶,和丙酮酸激酶)由于线粒体功能障碍而在自闭症儿童和对照组之间。
Mitochondrial dysfunction in autism leads to impair the mitochondria\'s ability to synthesis adenosine triphosphate (ATP) by impairment citric acid cycle as well as increase anaerobic glycolysis. Aim - measuring and evaluating the levels of mitochondrial markers; including glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), malate dehydrogenase, and pyruvate kinase) in the autistic group and knowing the possibility of using these markers to diagnose children with autism spectrum disorder. A case-control study was done in the Al-Zahraa Teaching Hospital (Kut City, Iraq) on 100 Iraqi children (male and female), between (April 2023 and January 2024). Their ages ranged between 3 and 9 years. Among them were 50 patients enrolled as autistic group and 50 healthy enrolled as control group. Blood samples were collected and bioassays for GOT, GPT, pyruvate kinase, and malate dehydrogenase were measured by ELISA technique. The autistic group showed that the urine GOT, urine GPT, serum malate, and serum pyruvate levels in the ASD group was significantly higher (P<0.001) than the control group. The ROC analysis showed that urine GOT, urine GOT, serum malate and serum pyruvate had an accuracy level of (81%,71%,77%, and 80 %) and the area under the curve (AUC) was > 0.7 (0.8),0.7, 0.7(0.76), and 0.7(0.8) thus urine GOT, urine GPT, serum, malate, and serum pyruvate are a valid diagnostic marker. There was a significant difference in the mean urine and serum concentrations of mitochondrial markers (GOT, GPT, malate dehydrogenase, and pyruvate kinase) between autistic children and the control group due to mitochondrial dysfunction.