目的:为了确定实际的乙型肝炎病毒(HBV)感染率,隐匿性HBV感染率(OBI),新疆农村牧区青少年人群OBI病毒S基因的分子进化特征。
方法:对农牧区青少年人群进行横断面问卷调查。静脉血液样本(3-5毫升)是从位于盘房沟乡的三所中心学校的合资格学生中收集的,水溪沟村,和苗尔沟村,都在乌鲁木齐县,在九年强制制度中。采用了群体中的聚类抽样,并获得了参与学生的知情同意书。通过电化学发光对所有血清样品进行乙型肝炎表面抗原(HBsAg)的定性测试。随后,通过巢式聚合酶链反应(PCR)扩增HBVS基因,纯化阳性PCR产物;然后扩增目标基因序列。使用MEGA11软件进行靶基因序列的分子进化表征。
结果:总体而言,纳入1712名受试者。HBsAg携带率和OBI感染率分别为1.93%(33/1712)和6.13%(103/1679),分别。HBsAg(-)样品包括103个OBI菌株,其中B基因型菌株占80.58%(83/103;1例ayw1血清型和82例adw2血清型),C基因型菌株占14.56%(15/103;adw2血清型1例,adrq+血清型14例),D基因型菌株占4.85%(5/103;adw2血清型1例,ayw2血清型4例)。在以下基因的“a”决定区检测到突变:P127S,G130R,和N146S(B基因型OBI菌株);T126I和T143S(C基因型OBI菌株);T126I,P127S,F134Y,和T143S(D基因型OBI菌株)。
结论:一定比例的年轻人感染了OBI菌株。OBI菌株的B基因型是可能的显性基因型。OBI菌株在“a”决定区具有氨基酸突变,它们的抗原性和免疫原性可能会发生变化。必须更加注意防止由于OBI引起的问题。
To determine the actual hepatitis B virus (HBV) infection rate, occult HBV infection (OBI) rate, and molecular evolutionary characteristics of the OBI virus S gene in the adolescent population living in rural and pastoral areas of Xinjiang Province.
A cross-sectional questionnaire survey was conducted among the adolescent population living in the farming and herding areas. Venous blood samples (3-5 mL) were collected from eligible students in three central schools located in Banfanggou Township, Shuixigou Village, and Miaolgou Village, all in Urumqi County, in the nine-year compulsory system. Clustersampling in a population was adopted, and informed consent was obtained from the participating students. All serum samples were qualitatively tested for hepatitis B surface antigen (HBsAg) by electrochemiluminescence. Subsequently, the HBV S gene was amplified by nested polymerase chain reaction (PCR), and the positive PCR products were purified; the target gene sequences were then amplified. Molecular evolutionary characterization of the target gene sequences was performed using MEGA 11software.
Overall, 1712 subjects were enrolled. The HBsAg carrier rate and OBI infection rate were 1.93% (33/1712) and 6.13% (103/1679), respectively. HBsAg (-) samples included 103 OBI strains, of which B-genotype strains accounted for 80.58% (83/103; 1 case of ayw1 serotype and 82 cases of adw2 serotype), C-genotype strains accounted for 14.56% (15/103; 1 case of adw2 serotype and 14 cases of adrq+serotype), and D-genotype strains accounted for 4.85% (5/103; 1 case of adw2 serotype and 4 cases of ayw2 serotype). Mutations were detected in the \"a\" determinant region of the following genes: P127S, G130R, and N146S (B-genotype OBI strains); T126I and T143S (C-genotype OBI strains); T126I, P127S, F134Y, and T143S (D-genotype OBI strains).
A certain proportion of young people are infected with OBI strains. The B-genotype of OBI strains is the possible dominant genotype. OBI strains have amino acid mutations in the \"a\" determinant region, and they are likely to undergo a change in their antigenicity and immunogenicity. More attention must be paid to prevent problems due to OBI.