迄今为止,近四分之一的结直肠癌(CRC)患者发生肝转移(CRCLM),其侵袭性可以与确定的组织病理学生长模式(HGP)相关。从CRCLM内的三个主要HGP来看,替代的HGP显得特别激进,以增强的肿瘤细胞运动和血管共选择为特征。这里,我们研究了钙和整合素结合蛋白1(CIB1)的表达之间的相关性,广泛表达的基因涉及各种细胞过程,包括迁移和粘附,原发性CRC患者的无病生存期(DFS)和总生存期(OS)。此外,我们探讨了CRCLM不同HGPs与CIB1表达的相关性。蛋白质组学分析用于评估697名原发性CRC患者队列中的CIB1表达。此外,单细胞和空间RNA测序数据集,使用公开的批量测序数据评估CRCLM中CIB1的表达。通过福尔马林固定的石蜡包埋的免疫组织化学染色进一步验证了计算机数据。我们观察到高CIB1表达与较差的DFS和OS独立相关,不管国际联盟对抗癌症阶段,性别,或年龄。此外,侵袭性替代CRCLMHGP与高CIB1表达显著相关。我们的发现表明CIB1水平与CRC的临床侵袭性之间存在相关性。此外,CIB1可能是对HGPCRCLM进行分层的新标记。
To date, nearly one-quarter of colorectal cancer (CRC) patients develop liver metastases (CRCLM), and its aggressiveness can be correlated to defined histopathological growth patterns (HGP). From the three main HGPs within CRCLM, the replacement HGP emerges as particularly aggressive, characterized by heightened tumor cell motility and vessel co-option. Here, we investigated the correlation between the expression of calcium- and integrin-binding protein 1 (CIB1), a ubiquitously expressed gene involved in various cellular processes including migration and adhesion, and disease-free (DFS) and overall survival (OS) in primary CRC patients. Additionally, we explored the correlation between CIB1 expression and different HGPs of CRCLM. Proteomic analysis was used to evaluate CIB1 expression in a cohort of 697 primary CRC patients. Additionally, single-cell and spatial RNA-sequencing datasets, along with publicly available bulk sequencing data were used to evaluate CIB1 expression in CRCLM. In silico data were further validated by formalin-fixed paraffin-embedded immunohistochemical stainings. We observed that high CIB1 expression is independently associated with worse DFS and OS, regardless of Union Internationale Contre le Cancer stage, gender, or age. Furthermore, the aggressive replacement CRCLM HGP is significantly associated with high CIB1 expression. Our findings show a correlation between CIB1 levels and the clinical aggressiveness of CRC. Moreover, CIB1 may be a novel marker to stratify HGP CRCLM.