D-塔格糖是一种有价值的稀有糖,具有潜在的健康益处,例如抗肥胖,低热量,益生元,和抗癌。然而,其生产主要依靠化学或酶催化。在这里,使用自组装方法在室温下在水性体系中开发了钴基金属有机骨架(MOF)。通过原位包封过程将L-阿拉伯糖异构酶(L-AI)固定到该独特的MOF中。MOF制剂的形态和结构方面通过不同的分析技术,如扫描电子显微镜(SEM),能量色散X射线光谱(EDX),共聚焦激光扫描显微镜(CLSM),傅里叶变换红外光谱(FT-IR),和X射线衍射(XRD)。此外,热重分析(TGA)表明L-AI@MOF具有较高的热稳定性。重要的是,固定化催化剂的催化效率(kcat/Km)提高了3.22mM-1s-1,周转数(kcat)提高了57.32s-1。L-AI@MOF可有效催化D-半乳糖合成D-塔格糖,直至多相催化中异构化的平衡水平(〜50%)。有趣的是,发现L-AI@MOF稳定且可重复使用超过五个循环,在催化过程中不需要额外的金属离子。因此,在MOF系统中稳定的L-AI表现出更高的催化活性和可持续合成稀有糖D-塔格糖的潜在指导。
D-tagatose is a valuable rare sugar with potential health benefits such as antiobesity, low-calorie, prebiotic, and anticancer. However, its production is mainly depending on chemical or enzymatic catalysis. Herein, a cobalt-based metal-organic framework (MOF) was developed at room temperature in an aqueous system using a self-assembly method. The L-arabinose isomerase (L-AI) was immobilized into this unique MOF by an in situ encapsulation process. The morphology and structural aspects of the MOF preparations were characterized by different analytical techniques such as scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), confocal laser scanning microscopy (CLSM), Fourier transform infrared spectroscopy (FT-IR), and X-Ray diffraction (XRD). Moreover, thermogravimetric analysis (TGA) suggested the high thermal stability of the L-AI@MOF. Significantly, the immobilized catalyst exhibited enhanced catalytic efficiency (kcat/Km) of 3.22 mM-1 s-1 and improved turnover number (kcat) of 57.32 s-1. The L-AI@MOF efficiently catalyzes the synthesis of D-tagatose from D-galactose up to the equilibrium level (~ 50%) of isomerization in heterogeneous catalysis. Interestingly, L-AI@MOF was found stable and reusable for more than five cycles without the requirement of additional metal ions during catalysis. Thus, L-AI stabilized in the MOF system demonstrated a higher catalytic activity and potential guidance for the sustainable synthesis of rare sugar D-tagatose.