■前列腺癌(PCa),泌尿生殖系统中最常见的恶性肿瘤之一,以远处转移和去势抵抗前列腺癌(CRPC)的发展为特征,这是预后不良的主要决定因素。目前PCa的治疗方法主要包括手术和内分泌治疗。但管理远处转移和CRPC的有效策略仍然有限.
■我们利用qPCR,WB,和其他方法来测量各自蛋白质的表达水平,同时评估脂质代谢以验证FATP5在脂质代谢中的作用。此外,我们采用生物信息学分析和WB技术来探索相应的机制。
■在这项研究中,我们对临床样本和公共数据库进行了分析,以确定FATP5的差异表达,并进一步研究了其与临床结局的相关性.通过生化和功能实验,我们阐明了FATP5促进PCa进展的潜在潜在机制.我们的研究结果表明,FATP5的特异性上调显着增强增殖,迁移,和PCa细胞系的侵袭,同时还调节PCa的脂质代谢。机械上,FATP5的表达与Hippo信号通路密切相关,因为它通过抑制AMPK并促进β-catenin和RHOA的激活来促进YAP1的核积累。此外,FATP5的转录是由TEAD4介导的,这种转录激活需要YAP1的参与。
■FATP5在前列腺癌中高表达,可以增强前列腺癌的生物学活性和脂质代谢。我们还阐明了FATP5受Hippo信号通路调节。这为前列腺癌的医治供给了新的潜伏靶点。
UNASSIGNED: Prostate cancer (PCa), one of the most prevalent malignant tumors in the genitourinary system, is characterized by distant metastasis and the development of castration-resistant prostate cancer (CRPC), which are major determinants of poor prognosis. Current treatment approaches for PCa primarily involve surgery and endocrine therapy, but effective strategies for managing distant metastasis and CRPC remain limited.
UNASSIGNED: We utilized qPCR, WB, and other methods to measure the expression levels of respective proteins, concurrently assessing lipid metabolism to validate the role of FATP5 in lipid metabolism. Additionally, we employed bioinformatics analysis and WB techniques to explore the corresponding mechanisms.
UNASSIGNED: In this study, we conducted an analysis of clinical samples and public databases to identify differential expression of FATP5 and further investigated its association with clinical outcomes. Through biochemical and functional experiments, we elucidated the potential underlying mechanisms by which FATP5 facilitates the progression of PCa. Our findings demonstrate that specific upregulation of FATP5 significantly enhances proliferation, migration, and invasion of PCa cell lines, while also modulating lipid metabolism in PCa. Mechanistically, the expression of FATP5 is closely associated with the
Hippo signaling pathway, as it promotes the nuclear accumulation of YAP1 by inhibiting AMPK and facilitating the activation of β-catenin and RHOA. Furthermore, the transcription of FATP5 is mediated by TEAD4, and this transcriptional activation requires the involvement of YAP1.
UNASSIGNED: FATP5 is highly expressed in prostate cancer and can enhance the biological activity and lipid metabolism of prostate cancer. We have also elucidated that FATP5 is regulated by the
Hippo signaling pathway. This provides a new potential target for the treatment of prostate cancer.